Characterization of the subcellular localization and nuclear import molecular mechanisms of herpes simplex virus 1 UL2

被引：15

作者：

Cai, Mingsheng ^{[1
,2
]}

Huang, Zebin ^{[3
]}

Liao, Zongmin ^{[1
]}

Chen, Tao ^{[1
]}

Wang, Ping ^{[1
]}

Jiang, Si ^{[1
]}

Chen, Daixiong ^{[1
,2
]}

Peng, Tao ^{[1
,2
]}

Bian, Yun ^{[4
]}

Hong, Gengde ^{[5
]}

Yang, Hang ^{[2
]}

Zeng, Zhancheng ^{[1
]}

Li, Xiaowei ^{[1
]}

Li, Meili ^{[1
,2
]}

机构：

[1] Guangzhou Med Univ, Sch Basic Med Sci, Sino French Hoffmann Inst, Dept Pathogen Biol & Immunol, Guangzhou 511436, Guangdong, Peoples R China

[2] Guangzhou Med Univ, Affiliated Hosp 2, Guangdong Prov Key Lab Allerg React & Clin Immuno, Guangzhou 510260, Guangdong, Peoples R China

[3] Univ Macau, Fac Hlth Sci, Taipa, Macao, Peoples R China

[4] Guangzhou Med Univ, Sch Life Sci, Guangzhou 510260, Guangdong, Peoples R China

[5] Guangzhou Med Univ, Clin Coll 3, Guangzhou 510260, Guangdong, Peoples R China

来源：

BIOLOGICAL CHEMISTRY | 2017年 / 398卷 / 04期

基金：

中国国家自然科学基金;

关键词：

HSV-1; UL2; importin; nuclear import; URACIL-DNA GLYCOSYLASE; VIRAL-DNA; PROTEIN IMPORT; EXPORT SIGNALS; TRANSPORT; ALPHA; ACCUMULATION; PATHWAY; TYPE-1; IDENTIFICATION;

D O I：

10.1515/hsz-2016-0268

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As a crucial protein, the herpes simplex virus 1 ( HSV-1) UL2 protein has been shown to take part in various stages of viral infection, nonetheless, its exact subcellular localization and transport molecular determinants are not well known thus far. In the present study, by using live cells fluorescent microscopy assay, UL2 tagged with enhanced yellow fluorescent protein was transiently expressed in live cells and showed a completely nuclear accumulation without the presence of other HSV-1 proteins. Moreover, the nuclear transport of UL2 was characterized to be assisted by multiple transport pathways through Ran-, importin alpha 1-, alpha 5-, alpha 7-, beta 1-and transportin-1 cellular transport receptors. Consequently, these results will improve understanding of UL2-mediated biological functions in HSV-1 infection cycles.

引用

页码：509 / 517

页数：9

共 39 条

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