Inactivation of the glutamate decarboxylase gene in Lactococcus lactis subsp cremoris

被引：31

作者：

Nomura, M ^{[1
]}

Kobayashi, M ^{[1
]}

Ohmomo, S ^{[1
]}

Okamoto, T ^{[1
]}

机构：

[1] Natl Inst Anim Ind, Dept Anim Prod, Tsukuba, Ibaraki 3050901, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 05期

关键词：

D O I：

10.1128/AEM.66.5.2235-2237.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lactococcus lactis subsp. lactis strains show glutamate decarboxylase activity, whereas L. lactis subsp. cremoris strains do not. The gadB gene encoding glutamate decarboxylase was detected in the L. lactis subsp. cremoris genome but was poorly expressed. Sequence analysis showed that the gene is inactivated by the frameshift mutation and encoded in a nonfunctional protein.

引用

页码：2235 / 2237

页数：3

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