A Visible-Light-Sensitive Caged Serotonin

被引：34

作者：

Cabrera, Ricardo ^{[1
,2
]}

Filevich, Oscar ^{[3
]}

Garcia-Acosta, Beatriz ^{[1
]}

Athilingam, Jegath ^{[4
,5
]}

Bender, Kevin J. ^{[4
,5
]}

Poskanzer, Kira E. ^{[4
,6
]}

Etchenique, Roberto ^{[1
]}

机构：

[1] Univ Buenos Aires, CONICET, Fac Ciencias Exactas & Nat, Dept Quim Inorgan Analit & Quim Fis,INQUIMAE, Ciudad Univ Pabellon 2,AR1428EHA, Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Ciclo Basico Comun, RA-1053 Buenos Aires, DF, Argentina

[3] Pontificia Univ Catolica Argentina, CONICET, Lab Neurobiol Mol, BIOMED, C1107AFB, Buenos Aires, DF, Argentina

[4] UCSF, Weill Inst Neurosci, Kavli Inst Fundamental Neurosci, San Francisco, CA 94143 USA

[5] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94143 USA

[6] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

来源：

ACS CHEMICAL NEUROSCIENCE | 2017年 / 8卷 / 05期

基金：

美国国家科学基金会;

关键词：

Ruthenium complex; serotonin; phototriggers; neurophysiology; RECEPTOR MODULATION; PYRAMIDAL NEURONS; GLUTAMATE; 2-PHOTON; RUTHENIUM; COMPLEX; ACTIVATION; EXPRESSION; RELEASE; SPINES;

D O I：

10.1021/acschemneuro.7b00083

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Serotonin, or 5-hydroxytryptamine (5HT), is an important neurotransmitter in the nervous system of both vertebrates and invertebrates. Deficits in 5HT signaling are responsible for many disabling psychiatric conditions, and its molecular machinery is the target of many pharmaceuticals. 5 We present a new 5HT phototrigger, the compound [Ru(bpy)(2)(PMe3)(5HT)](2+), where PMe3 is trimethylphosphine. As with other ruthenium-bipyridyl based caged compounds, [Ru(bpy)(2)(PMe3)(5HT)](2+) presents activity in the visible region of the spectrum. We characterize and discuss the photochemical properties of the caged compound, and demonstrate its use by modulating the excitability of mouse prefrontal principal neurons.

引用

页码：1036 / 1042

页数：7

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