Optimized dyeing conditions of immunoprotein with reactive dye Procion Blue MX-7RX

被引：9

作者：

Wang, Shwu-Jinng

Lin, Hsiu-Min

Wang, Xin-Ren

Hsiung, Kuang-Pin

Liu, Yung-Chuan ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 402, Taiwan

[2] Taiwan Unison Biotech, Dept Res & Dev, Chunan 350, Taiwan

来源：

ANALYTICAL BIOCHEMISTRY | 2007年 / 361卷 / 02期

关键词：

reactive dyes; immunochromatographic test; optimal reaction conditions; response surface methodology;

D O I：

10.1016/j.ab.2006.11.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For investigating the feasibility of using reactive dyes as an immunoassay marker, a dichlorine triazine dye, Procion Blue MX-7RX, was employed to stain the antibody against human serum albumin (anti-HSA). With the color intensity revealed in the immunochromatographic test strip as the objective variables, the optimal dyeing conditions were found as follows: pH 11.4, temperature 35.7 degrees C, molar ratio 188 (mol dye/mol antibody), and reaction time 45.6 min. The dyed-anti-HSA revealed a maximal color intensity of 8738 without apparent loss of antigen binding affinity. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：190 / 196

页数：7

共 29 条

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