Modular vaccine platform based on the norovirus-like particle

被引：27

作者：

Lampinen, Vili ^{[1
]}

Heinimaki, Suvi ^{[2
]}

Laitinen, Olli H. ^{[1
]}

Pesu, Marko ^{[1
,3
]}

Hankaniemi, Minna M. ^{[1
]}

Blazevic, Vesna ^{[2
]}

Hytoenen, Vesa P. ^{[1
,3
]}

机构：

[1] Tampere Univ, Fac Med & Hlth Technol, Tampere 33014, Finland

[2] Tampere Univ, Fac Med & Hlth Technol, Vaccine Dev & Immunol Vaccine Res Ctr, Tampere, Finland

[3] Fimlab Labs, Tampere, Finland

来源：

JOURNAL OF NANOBIOTECHNOLOGY | 2021年 / 19卷 / 01期

基金：

芬兰科学院;

关键词：

VLP; Vaccine; Influenza; SpyCatcher; Virus;

D O I：

10.1186/s12951-021-00772-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

BackgroundVirus-like particle (VLP) vaccines have recently emerged as a safe and effective alternative to conventional vaccine technologies. The strong immunogenic effects of VLPs can be harnessed for making vaccines against any pathogen by decorating VLPs with antigens from the pathogen. Producing the antigenic pathogen fragments and the VLP platform separately makes vaccine development rapid and convenient. Here we decorated the norovirus-like particle with two conserved influenza antigens and tested for the immunogenicity of the vaccine candidates in BALB/c mice.ResultsSpyTagged noro-VLP was expressed with high efficiency in insect cells and purified using industrially scalable methods. Like the native noro-VLP, SpyTagged noro-VLP is stable for months when refrigerated in a physiological buffer. The conserved influenza antigens were produced separately as SpyCatcher fusions in E. coli before covalent conjugation on the surface of noro-VLP. The noro-VLP had a high adjuvant effect, inducing high titers of antibody production against the antigens presented on its surface.ConclusionsThe modular noro-VLP vaccine platform presented here offers a rapid, convenient and safe method to present various soluble protein antigens to the immune system for vaccination and antibody production purposes.

引用

页数：14

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