UBE2S drives elongation of K11-linked ubiquitin chains by the Anaphase-Promoting Complex

被引：188

作者：

Wu, Tao ^{[1
]}

Merbl, Yifat ^{[1
]}

Huo, Ying ^{[2
]}

Gallop, Jennifer L. ^{[1
]}

Tzur, Amit ^{[1
]}

Kirschner, Marc W. ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA

[2] Jilin Univ, Sch Pharm, Changchun 130021, Jilin Province, Peoples R China

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 04期

关键词：

E2; enzyme; E3; ligase; proteasome; cell cycle; mitosis; CARRIER PROTEIN; POLYUBIQUITIN CHAINS; CONJUGATING ENZYMES; MITOTIC CYCLINS; APC; IDENTIFICATION; DEGRADATION; CHECKPOINT; DESTRUCTION; MECHANISM;

D O I：

10.1073/pnas.0912802107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Anaphase-Promoting Complex (APC) is an E3 ubiquitin ligase that regulates mitosis and G1 by sequentially targeting cell-cycle regulators for ubiquitination and proteasomal degradation. The mechanism of ubiquitin chain formation by APC and the resultant chain topology remains controversial. By using a single-lysine APC substrate to dissect the topology of ubiquitinated substrates, we find that APC-catalyzed ubiquitination has an intrinsic preference for the K11 linkage of ubiquitin that is essential for substrate degradation. K11 specificity is determined by an E2 enzyme, UBE2S/E2-EPF, that elongates ubiquitin chains after the substrates are pre-ubiquitinated by UbcH10 or UbcH5. UBE2S copurifies with APC; dominant-negative Ube2S slows down APC substrate degradation in functional cell-cycle extracts. We propose that Ube2S is a critical, unique component of the APC ubiquitination pathway.

引用

页码：1355 / 1360

页数：6

共 32 条

[1] UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1α turnover [J].

Alexandru, Gabriela ;

Graumann, Johannes ;

Smith, Geoffrey T. ;

Kolawa, Natalie J. ;

Fang, Ruihua ;

Deshaies, Raymond J. .

CELL, 2008, 134 (05) :804-816

[2] E2-C, a cyclin-selective ubiquitin carrier protein required for the destruction of mitotic cyclins [J].

Aristarkhov, A ;

Eytan, E ;

Moghe, A ;

Admon, A ;

Hershko, A ;

Ruderman, JV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :4294-4299

[3] Novel multiubiquitin chain linkages catalyzed by the conjugating enzymes E2(EPF) and RAD6 are recognized by 26 S proteasome subunit 5 [J].

Baboshina, OV ;

Haas, AL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (05) :2823-2831

[4] Global changes to the ubiquitin system in Huntington's disease [J].

Bennett, Eric J. ;

Shaler, Thomas A. ;

Woodman, Ben ;

Ryu, Kwon-Yul ;

Zaitseva, Tatiana S. ;

Becker, Christopher H. ;

Bates, Gillian P. ;

Schulman, Howard ;

Kopito, Ron R. .

NATURE, 2007, 448 (7154) :704-U11

[5] Inhibitory factors associated with anaphase-promoting complex/cylosome in mitotic checkpoint [J].

Braunstein, Ilana ;

Miniowitz, Shirly ;

Moshe, Yakir ;

Hershko, Avram .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (12) :4870-4875

[6]

CHEN ZJ, 1990, J BIOL CHEM, V265, P21835

[7] E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages [J].

Christensen, Devin E. ;

Brzovic, Peter S. ;

Klevit, Rachel E. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2007, 14 (10) :941-948

[8] UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit [J].

Garnett, Mathew J. ;

Mansfeld, Joerg ;

Godwin, Colin ;

Matsusaka, Takahiro ;

Wu, Jiahua ;

Russell, Paul ;

Pines, Jonathon ;

Venkitaraman, Ashok R. .

NATURE CELL BIOLOGY, 2009, 11 (11) :1363-U241

[9]

Ikeda F, 2008, EMBO REP, V9, P536, DOI 10.1038/embor.2008.93

[10] Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex [J].

Jin, Lingyan ;

Williamson, Adam ;

Banerjee, Sudeep ;

Philipp, Isabelle ;

Rape, Michael .

CELL, 2008, 133 (04) :653-665

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