Recovery of Proteolytic and Collagenolytic Activities from Viscera By-products of Rayfish (Raja clavata)

被引：22
作者：
Anxo Murado, Miguel [1 ]
del Pilar Gonzalez, Maria [1 ]
Antonio Vazquez, Jose [1 ]
机构：
[1] CSIC, Inst Invest Marinas, Grp Reciclado & Valorizac Mat Residuales, Vigo 36208, Galicia, Spain
来源：
MARINE DRUGS | 2009年 / 7卷 / 04期
关键词：
proteolytic and collagenolytic activity; by-products upgrading; rayfish viscera wastes; pH and temperature effects; mathematical modelling; SARDINOPS-SAGAX-CAERULEA; LACTIC-ACID BACTERIA; PROTEASE PRODUCTION; ALKALINE PROTEASES; PYLORIC CECA; PURIFICATION; FERMENTATION; TRYPSIN; GROWTH; WASTE;
D O I：
10.3390/md7040803
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
The aim of this work was to study the recovery of proteolytic and collagenolytic activities from rayfish (Raja clavata) viscera wastes. Initially, different parts of the gastrointestinal tract by-products (stomach, duodenum section including pancreas, final intestine) were evaluated. The extracts from proximal intestine yielded the highest values of both enzymatic activities. Optimal conditions for protease activity quantification were established at pH = 6, T = 40 degrees C and incubation time <= 20 min. The mathematical equation used to model the joint effect of pH and temperature led to maximum activity at pH = 8.66 and 59.4 degrees C, respectively. Overcooled acetone was found to be best option for recovery of enzymatic activities in comparison with ethanol, PEG-4000, ammonium sulphate and ultrafiltration system. Finally, a simple and systematic protocol of partial purification and total recovery of proteases and collagenases was defined.
引用
收藏
页码：803 / 815
页数：13
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