The obligate root parasite Orobanche cumana exhibits several rbcL sequences

被引：22

作者：

Delavault, P ^{[1
]}

Thalouarn, P ^{[1
]}

机构：

[1] Fac Sci & Tech, Grp Physiol & Pathol Vegetales, F-44322 Nantes 3, France

来源：

GENE | 2002年 / 297卷 / 1-2期

关键词：

gene transfer; parasitic plant; plastid genome; evolution;

D O I：

10.1016/S0378-1119(02)00868-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Orobanche species characterization using plastid sequences as molecular markers revealed that O. cumana contains at least two distinct rbcL sequences: one similar in size to the truncated rbcL pseudogene from O. cernua, a closely related species, and another with a size comparable to that of rbcL plastid genes from autotrophic plants. In this work, the nucleotide sequences of these two copies are reported and analysed. The organization of the O. cumana plastid genome was investigated using a long-distance PCR strategy in order to determine their localization. Because of the non-plastid localization of the rbcL larger copy, Southern blot and PCR chromosome-walking experiments were carried out to better characterize this transferred sequence and to identify its localization. Then the mode of multiple transfer of genetic information from plastid to nucleus and the concomitant plastid sequence disorganization and migration during parasitic plant evolution are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：85 / 92

页数：8

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