Acid pre-adaptation enhances virulence of Salmonella enterica serovar Typhimurium dam mutant

It is well established that success or failure of bacterial pathogens during infection relies upon its ability to overcome many lethal environments in the host such as acidity, osmolarity and bile salts. In the present study, we have studied the effects of acid adaptation on the virulence of Salmonella enterica serovar Typhimurium dam mutant. Our results indicated that LD50 of adapted strains were lower than those of control strains. Also, the in vivo assays have shown that the development of a systemic infection is slower for control strains than for adapted strains. In addition, the number of acid-adapted mutants colonizing spleen and liver is higher than control strains. Adhesion and invasion experiments were performed in order to compare the pathogenicity of Salmonella. No significant differences were shown between pre-treated and non-adapted strains. According to these results, we report that acid adaptation of Salmonella enterica serovar Typhimurium dam mutants can increase their in vivo virulence in mice. (C) 2008 Elsevier Masson SAS. All fights reserved.