Rhythmic pore dynamics in a shrinking lipid vesicle

被引：36

作者：

Hamada, Tsutomu ^{[1
]}

Hirabayashi, Yuichi ^{[1
]}

Ohta, Takao ^{[2
]}

Takagi, Masahiro ^{[1
]}

机构：

[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Nomi, Ishikawa 9231292, Japan

[2] Kyoto Univ, Grad Sch Sci, Dept Phys, Kyoto 6068502, Japan

来源：

PHYSICAL REVIEW E | 2009年 / 80卷 / 05期

关键词：

biomechanics; biomembranes; cellular biophysics; lipid bilayers; oscillations; surfactants; TRANSIENT PORES; PHOSPHOLIPID-VESICLES; BUDDING TRANSITIONS; BILAYER VESICLES; FLUID MEMBRANES; GIANT VESICLES; LIPOSOMES; SOLUBILIZATION; FLUCTUATIONS; DEFORMATION;

D O I：

10.1103/PhysRevE.80.051921

中图分类号：

O35 [流体力学]; O53 [等离子体物理学];

学科分类号：

070204 ; 080103 ; 080704 ;

摘要：

The rhythmic motion of membrane pore behavior under nonequilibrium conditions was studied. Application of the surfactant triton X-100 (TX-100) caused lipid vesicles to exhibit two types of shrinking dynamics with pore generation, which depended on both the size of the vesicles and the concentration of added TX-100. Small vesicles and the addition of a low concentration of TX-100 resulted in rhythmic-pore dynamics, where a transient pore was generated within a vesicle in a repetitive manner. In contrast, large vesicles and a high concentration of TX-100 led to continuous-pore dynamics, where the vesicle maintained an open pore during the shrinking process. In the rhythmic-pore membrane, long-cycle oscillation was observed with large vesicles and a low concentration TX-100. The period of one cycle decreased with a decrease in the vesicle size and an increase in the TX-100 concentration. We discuss the mechanism of these trends by considering the elastic free energy of the membrane.

引用

页数：7

共 51 条

[1] Mixed micelles and other structures in the solubilization of bilayer lipid membranes by surfactants [J].

Almgren, M .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2000, 1508 (1-2) :146-163

[2] Phase behavior of multicomponent membranes: Experimental and computational techniques [J].

Bagatolli, Luis ;

Kumar, P. B. Sunil .

SOFT MATTER, 2009, 5 (17) :3234-3248

[3] Dynamic excitations in membranes induced by optical tweezers [J].

Bar-Ziv, R ;

Moses, E ;

Nelson, P .

BIOPHYSICAL JOURNAL, 1998, 75 (01) :294-320

[4] Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension [J].

Baumgart, T ;

Hess, ST ;

Webb, WW .

NATURE, 2003, 425 (6960) :821-824

[5] Steady to unsteady dynamics of a vesicle in a flow -: art. no. 011906 [J].

Beaucourt, J ;

Rioual, F ;

Séon, T ;

Biben, T ;

Misbah, C .

PHYSICAL REVIEW E, 2004, 69 (01) :17

[6] Transient pores in stretched vesicles: role of leak-out [J].

Brochard-Wyart, F ;

de Gennes, PG ;

Sandre, O .

PHYSICA A, 2000, 278 (1-2) :32-51

[7] Deformation of giant lipid bilayer vesicles in shear flow [J].

de Haas, KH ;

Blom, C ;

van den Ende, D ;

Duits, MHG ;

Mellema, J .

PHYSICAL REVIEW E, 1997, 56 (06) :7132-7137

[8] VESICLE-MICELLE STRUCTURAL TRANSITIONS OF PHOSPHOLIPID-BILAYERS AND SODIUM DODECYL-SULFATE [J].

DELAMAZA, A ;

PARRA, JL .

LANGMUIR, 1995, 11 (07) :2435-2441

[9] Phase Diagram of Single Vesicle Dynamical States in Shear Flow [J].

Deschamps, J. ;

Kantsler, V. ;

Steinberg, V. .

PHYSICAL REVIEW LETTERS, 2009, 102 (11)

[10] A practical guide to giant vesicles. Probing the membrane nanoregime via optical microscopy [J].

Dimova, Rumiana ;

Aranda, Said ;

Bezlyepkina, Natalya ;

Nikolov, Vesselin ;

Riske, Karin A. ;

Lipowsky, Reinhard .

JOURNAL OF PHYSICS-CONDENSED MATTER, 2006, 18 (28) :S1151-S1176

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