Fluorescent Proteins for Live Cell Imaging: Opportunities, Limitations, and Challenges

被引：192
作者：
Wiedenmann, Joerg [1 ]
Oswald, Franz [2 ]
Nienhaus, Gerd Ulrich [3 ,4 ,5 ,6 ]
机构：
[1] Univ Southampton, Natl Oceanog Ctr, Southampton SO14 3ZH, Hants, England
[2] Univ Ulm, Dept Internal Med, D-7900 Ulm, Germany
[3] Univ Karlsruhe, Inst Appl Phys, Karlsruhe, Germany
[4] Univ Karlsruhe, Ctr Funct Nanostruct, Karlsruhe, Germany
[5] Univ Ulm, Inst Biophys, Ulm, Germany
[6] Univ Illinois, Dept Phys, Urbana, IL 61801 USA
来源：
IUBMB LIFE | 2009年 / 61卷 / 11期
关键词：
live cell imaging; coral fluorescent proteins; GFP; RFP; monomeric red fluorescent protein; far red fluorescent protein; photoconversion; photoactivation; super resolution microscopy; GFP-LIKE PROTEINS; ANEMONE ENTACMAEA-QUADRICOLOR; ASSISTED LIGHT INACTIVATION; TO-RED CONVERSION; STRUCTURAL BASIS; MONOMERIC RED; CRYSTAL-STRUCTURE; BLUE-LIGHT; STATE STRUCTURE; MARKER PROTEIN;
D O I：
10.1002/iub.256
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of. the glowing probes. (C) 2009 IUBMB IUBMB Life, 61(11): 1029-1042, 2009
引用
收藏
页码：1029 / 1042
页数：14
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