DNA - Gold Nanoparticles Designed for mRNA Sensing in Cells: Imaging of the Gold Nanoparticles Using Two Photon Photoluminescence Spectroscopy

被引：0
作者：
Kyriazi, Maria-Eleni [1 ]
Muskens, Otto L. [1 ,2 ]
Kanaras, Antonios G. [1 ,2 ]
机构：
[1] Univ Southampton, Sch Phys & Astron, Southampton SO17 1BJ, Hants, England
[2] Univ Southampton, Inst Life Sci, Southampton SO17 1BJ, Hants, England
来源：
COLLOIDAL NANOPARTICLES FOR BIOMEDICAL APPLICATIONS XIV | 2019年 / 10892卷
基金：
英国生物技术与生命科学研究理事会;
关键词：
DNA; mRNA; nanoparticles; colloids; drugs; cancer; TPPL; SIZE; NANORODS; ABSORPTION; PLATFORM; PROBE;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Recently DNA - coated gold nanoparticles have emerged as ideal tools for the detection of mRNA in cells using dye modified oligonucleotides. However, the tracking of the gold core has been hindered by the small size of the particle core. In this work we utilize a home built set up and 43 nm DNA - coated spherical gold nanoparticles for the simultaneous imaging of mRNA detection using fluorescence microscopy and the gold nanoparticle core using two photon photoluminescence (TPPL).
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页数：10
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  • [1] Kinetically Controlled Seeded Growth Synthesis of Citrate-Stabilized Gold Nanoparticles of up to 200 nm: Size Focusing versus Ostwald Ripening
    Bastus, Neus G.
    Comenge, Joan
    Puntes, Victor
    [J]. LANGMUIR, 2011, 27 (17) : 11098 - 11105
  • [2] Observation of intrinsic size effects in the optical response of individual gold nanoparticles
    Berciaud, S
    Cognet, L
    Tamarat, P
    Lounis, B
    [J]. NANO LETTERS, 2005, 5 (03) : 515 - 518
  • [3] Spherical Nucleic Acids
    Cutler, Joshua I.
    Auyeung, Evelyn
    Mirkin, Chad A.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (03) : 1376 - 1391
  • [4] Two-photon luminescence imaging of cancer cells using molecularly targeted gold nanorods
    Durr, Nicholas J.
    Larson, Timothy
    Smith, Danielle K.
    Korgel, Brian A.
    Sokolov, Konstantin
    Ben-Yakar, Adela
    [J]. NANO LETTERS, 2007, 7 (04) : 941 - 945
  • [5] Shape-Dependent Two-Photon Photoluminescence of Single Gold Nanoparticles
    Gao, Nengyue
    Chen, Yang
    Li, Lin
    Guan, Zhenping
    Zhao, Tingting
    Zhou, Na
    Yuan, Peiyan
    Yao, Shao Q.
    Xu, Qing-Hua
    [J]. JOURNAL OF PHYSICAL CHEMISTRY C, 2014, 118 (25) : 13904 - 13911
  • [6] Selective killing of cells triggered by their mRNA signature in the presence of smart nanoparticles
    Heuer-Jungemann, Amelie
    El-Sagheer, Afaf H.
    Lackie, Peter M.
    Brown, Tom
    Kanaras, Antonios G.
    [J]. NANOSCALE, 2016, 8 (38) : 16857 - 16861
  • [7] Gold nanoparticles and fluorescently-labelled DNA as a platform for biological sensing
    Heuer-Jungemann, Amelie
    Harimech, Pascal K.
    Brown, Tom
    Kanaras, Antonios G.
    [J]. NANOSCALE, 2013, 5 (20) : 9503 - 9510
  • [8] The Role Radius of Curvature Plays in Thiolated Oligonucleotide Loading on Gold Nanoparticles
    Hill, Haley D.
    Millstone, Jill E.
    Banholzer, Matthew J.
    Mirkin, Chad A.
    [J]. ACS NANO, 2009, 3 (02) : 418 - 424
  • [9] Gold nanoparticles: Optical properties and implementations in cancer diagnosis and photothermal therapy
    Huang, Xiaohua
    El-Sayed, Mostafa A.
    [J]. JOURNAL OF ADVANCED RESEARCH, 2010, 1 (01) : 13 - 28
  • [10] Maximizing DNA loading on a range of gold nanoparticle sizes
    Hurst, Sarah J.
    Lytton-Jean, Abigail K. R.
    Mirkin, Chad A.
    [J]. ANALYTICAL CHEMISTRY, 2006, 78 (24) : 8313 - 8318
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