Isolation of ten microsatellite loci in an EST library of the phytopathogenic fungus Puccinia striiformis f.sp tritici

被引:43
作者
Bahri, Bochra [1 ]
Leconte, Marc [1 ]
de Vallavieille-Pope, Claude [1 ]
Enjalbert, Jerome [1 ]
机构
[1] BIOGER CPP INRA AgroParisTech, UMR 1290, Thiverval Grignon, France
关键词
Basidiomycota; Clonal biotrophic fungus; EST library; Wheat yellow (stripe) rust; MARKERS;
D O I
10.1007/s10592-008-9752-5
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
We report the characterization of ten microsatellite markers in the fungus Puccinia striiformis f.sp. tritici, responsible for yellow rust disease on wheat. A published EST library was scanned for microsatellite motives, and over 15 selected EST sequences, 13 were successfully amplified and ten exhibited polymorphism over an international collection of 43 isolates. These new microsatellites, added to the few previously published ones, provide a sufficient set of markers to perform population genetic studies.
引用
收藏
页码:1425 / 1428
页数:4
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