Antistaphylococcal and Neutrophil Chemotactic Injectable κ-Carrageenan Hydrogel for Infectious Wound Healing

被引：27

作者：

Muthuswamy, Shiva ^{[1
]}

Viswanathan, Aparna ^{[1
]}

Yegappan, Ramanathan ^{[1
]}

Selvaprithiviraj, Vignesh ^{[1
]}

Vasudevan, Anil Kumar ^{[2
]}

Biswas, Raja ^{[1
]}

Jayakumar, Rangasamy ^{[1
]}

机构：

[1] Amrita Vishwa Vidyapeetham, Amrita Inst Med Sci & Res Ctr, Ctr Nanosci & Mol Med, Kochi 682041, Kerala, India

[2] Amrita Vishwa Vidyapeetham, Amrita Inst Med Sci & Res Ctr, Dept Microbiol, Kochi 682041, Kerala, India

来源：

ACS APPLIED BIO MATERIALS | 2019年 / 2卷 / 01期

关键词：

chitosan-treated serum; kappa-carrageenan; octenidine dihydrochloride; infected wounds; wound healing;

D O I：

10.1021/acsabm.8b00625

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Staphylococcus aureus wound infection is a major concern due to the resistance of S. aureus to topical antibiotics and capacity to inhibit neutrophil migration at the infection site. To overcome these problems, we have developed 0.01% (v/v) octenidine dihydrochloride (Oct) and 0.5% (w/w) chitosan-treated serum (CTS) containing 1.5% (w/v) kappa-carrageenan hydrogel (kappa C). Oct is an antiseptic agent, against which no resistance is reported so far, and CTS has neutrophilic attractant properties. The prepared Oct-CTS-kappa C hydrogel is injectable and biocompatible. Using in vitro experiments, we demonstrated CTS can induce the migration of polymorphonuclear neutrophils (PMNs) and fibroblasts that can facilitate tissue regeneration at a wound site. In vitro release studies revealed a sustained release of Oct and serum proteins from the Oct-CTS-kappa C hydrogel. Antibacterial properties of developed hydrogels were tested against S. aureus and its clinical isolates. Further, the in vivo antibacterial efficacy of the prepared hydrogel was evaluated in an S. aureus-infected Sprague-Dawley (SD) rat wound. Both in vitro and in vivo studies showed that the Oct-CTS-kappa C hydrogel inhibited S. aureus growth. Thus, the developed Oct-CTS-kappa C hydrogel can be potentially exploited for S. aureus-infected wound healing.Y

引用

页码：378 / 387

页数：10

共 44 条

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