CytoMatrix for a reliable and simple characterization of lung cancer stem cells from malignant pleural effusions

被引：29

作者：

Bruschini, Sara ^{[1
]}

di Martino, Simona ^{[2
]}

Pisanu, Maria Elena ^{[3
]}

Fattore, Luigi ^{[4
]}

De Vitis, Claudia ^{[5
]}

Laquintan, Alentina ^{[2
]}

Buglioni, Simonetta ^{[2
]}

Tabbi, Eugenio ^{[5
]}

Cerri, Andrea ^{[6
]}

Visca, Paolo ^{[2
]}

Alessandrin, Gabriele ^{[7
]}

Facciolo, Francesco ^{[7
]}

Napoli, Christian ^{[8
]}

Trombetta, Marcella ^{[9
]}

Santoro, Antonio ^{[10
]}

Crescenzi, Anna ^{[11
]}

Ciliberto, Gennaro ^{[12
]}

Mancini, Rita ^{[5
]}

机构：

[1] Magna Graecia Univ Catanzaro, Dept Expt & Clin Med, Catanzaro, Italy

[2] IRCCS, Regina Elena Natl Canc Inst, Phatol Unit, Rome, Italy

[3] Ist Super Sanita, High Resolut NMR Unit, Core Facil, Rome, Italy

[4] Sapienza Univ Rome, Fdn Cenci Bolognetti, Dept Clin & Mol Med, Lab Affiliated Ist Pasteur Italia, Rome, Italy

[5] Sapienza Univ Rome, Dept Clin & Mol Med, I-00161 Rome, Italy

[6] IRCCS, Regina Elena Natl Canc Inst, Preclin Models & New Therapeut Agents Unit, Rome, Italy

[7] IRCCS, Regina Elena Natl Canc Inst, Thorac Surg Unit, Rome, Italy

[8] Sapienza Univ Rome, Dept Med Surg Sci & Translat Med, Rome, Italy

[9] Univ Campus Biomed, Tissue Engn & Chem Engn Lab, Dept Engn, Rome, Italy

[10] UCS Diagnost Srl, Rome, Italy

[11] Univ Hosp Campus Biomed Rome, Sect Pathol, Rome, Italy

[12] IRCCS, Regina Elena Natl Canc Inst, Sci Direct, Rome, Italy

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2020年 / 235卷 / 03期

关键词：

cancer stem cells; CytoMatrix; lung cancer; MPEs; SCD1; CLINICAL-PRACTICE GUIDELINES; CISPLATIN; MARKERS; IDENTIFICATION; EXPRESSION; DIAGNOSIS; PULMONARY; TAZ;

D O I：

10.1002/jcp.29121

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cancer stem cells (CSCs) are a subpopulation with the properties of extensive self-renewal, capability to generate differentiated cancer cells and resistance to therapies. We have previously shown that malignant pleural effusions (MPEs) from patients with non-small-cell lung cancer (NSCLC) represent a valuable source of cancer cells that can be grown as three-dimensional (3D) spheroids enriched for stem-like features, which depend on the activation of the Yes-associated protein-transcriptional coactivator with PDZ-binding motif (YAP-TAZ)/Wnt-beta catenin/stearoyl-CoA desaturase 1 (SCD1) axis. Here, we describe a novel support, called CytoMatrix, for the characterization of limited amounts of cancer cells isolated from MPEs of patients with NSCLC. Our results show that this synthetic matrix allows an easy and fast characterization of several epithelial cellular markers. The use of CytoMatrix to study CSCs subpopulation confirms that SCD1 protein expression is enhanced in 3D spheroids when compared with 2D adherent cell cultures. YAP/TAZ nuclear-cytoplasmic distribution analysed by CytoMatrix in 3D spheroids is highly heterogeneous and faithfully reproduces what is observed in tumour biopsies. Our results confirm and extend the robustness of our workflow for the isolation and phenotypic characterization of primary cancer cells derived from the lung MPEs and underscore the role of SCD1.

引用

页码：1877 / 1887

页数：11

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[1] Mechanisms of chemoresistance in cancer stem cells [J].

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Acunzo, Mario ;

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Nigita, Giovanni ;

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Lagana, Alessandro ;

Zanesi, Nicola ;

Fadda, Paolo ;

Fassan, Matteo ;

Rizzotto, Lara ;

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Coppola, Vincenzo ;

Croce, Carlo M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (21) :E4203-E4212

[3] Tumor Evolution as a Therapeutic Target [J].

Amirouchene-Angelozzi, Nabil ;

Swanton, Charles ;

Bardelli, Alberto .

CANCER DISCOVERY, 2017, 7 (08) :805-817

[4] The Malignant Pleural Effusion as a Model to Investigate Intratumoral Heterogeneity in Lung Cancer [J].

Basak, Saroj K. ;

Veena, Mysore S. ;

Oh, Scott ;

Huang, Ge ;

Srivatsan, Eri ;

Min Huang ;

Sharma, Sherven ;

Batra, Raj K. .

PLOS ONE, 2009, 4 (06)

[5] Targeting T Cell Co-receptors for Cancer Therapy [J].

Callahan, Margaret K. ;

Postow, Michael A. ;

Wolchok, Jedd D. .

IMMUNITY, 2016, 44 (05) :1069-1078

[6] Immunostaining in Lung Cancer for the Clinician Commonly Used Markers for Differentiating Primary and Metastatic Pulmonary Tumors [J].

Carney, John M. ;

Kraynie, Alyssa M. ;

Roggli, Victor L. .

ANNALS OF THE AMERICAN THORACIC SOCIETY, 2015, 12 (03) :429-435

[7] Cancer stem cells as targets for immunotherapy [J].

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IMMUNOLOGY, 2018, 153 (03) :304-314

[8] Prospective identification of tumorigenic prostate cancer stem cells [J].

Collins, AT ;

Berry, PA ;

Hyde, C ;

Stower, MJ ;

Maitland, NJ .

CANCER RESEARCH, 2005, 65 (23) :10946-10951

[9] The Hippo Transducer TAZ Confers Cancer Stem Cell-Related Traits on Breast Cancer Cells [J].

Cordenonsi, Michelangelo ;

Zanconato, Francesca ;

Azzolin, Luca ;

Forcato, Mattia ;

Rosato, Antonio ;

Frasson, Chiara ;

Inui, Masafumi ;

Montagner, Marco ;

Parenti, Anna R. ;

Poletti, Alessandro ;

Daidone, Maria Grazia ;

Dupont, Sirio ;

Basso, Giuseppe ;

Bicciato, Silvio ;

Piccolo, Stefano .

CELL, 2011, 147 (04) :759-772

[10] Identification and expansion of the tumorigenic lung cancer stem cell population [J].

Eramo, A. ;

Lotti, F. ;

Sette, G. ;

Pilozzi, E. ;

Biffoni, M. ;

Di Virgilio, A. ;

Conticello, C. ;

Ruco, L. ;

Peschle, C. ;

De Maria, R. .

CELL DEATH AND DIFFERENTIATION, 2008, 15 (03) :504-514

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