Ginsenoside Rg1 Protects Rat Cardiomyocyte From Hypoxia/Reoxygenation Oxidative Injury Via Antioxidant and Intracellular Calcium Homeostasis

被引：114

作者：

Zhu, Dan ^{[1
,2
]}

Wu, Lei ^{[3
]}

Li, Chun-Rong ^{[1
]}

Wang, Xiao-Wei ^{[1
]}

Ma, Yong-Jie ^{[1
]}

Zhong, Zhi-yin ^{[1
]}

Zhao, Hai-Bao ^{[1
]}

Cui, Jian ^{[2
]}

Xun, Si-Fan ^{[2
]}

Huang, Xiu-Lan ^{[2
]}

Zhou, Zhe ^{[1
]}

Wang, Sheng-Qi ^{[1
]}

机构：

[1] Beijing Inst Radiat Med, Beijing 100850, Peoples R China

[2] Minzu Univ China, Inst Chinese Tradit Natl Med, Beijing 100081, Peoples R China

[3] Peking Univ, Hosp 3, Inst Cardiovasc Dis, Beijing 100083, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2009年 / 108卷 / 01期

关键词：

GINSENOSIDE Rg1; HYPOXIA/REOXYGENATION; ANTIOXIDANT; REACTIVE OXYGEN SPECIES; Ca2+ OVERLOAD; PANAX-NOTOGINSENG SAPONINS; INDUCED APOPTOSIS; FREE-RADICALS; STRESS; REPERFUSION; GLUTATHIONE; MECHANISM; ISCHEMIA; MYOCYTES; HYPOXIA;

D O I：

10.1002/jcb.22233

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ginsenoside Rg1 is a major active ingredient of Panax notoginseng radix which has demonstrated a number of pharmacological actions including a cardioprotective effect in vivo. This study investigated the protective effect and mechanism of ginsenoside Rg1 in cardiomyocytes hypoxia/reoxygenation (H/R) model. Pretreatment with ginsenoside Rg1 (60-120 mu M) reduced lactate dehydrogenase release and increased cell viability in a dose-dependent manner. Fluorescence analysis demonstrated ginsenoside Rg1 reduced intracellular ROS and suppressed the intracellular [Ca2+] level. Cell lysate detected an increase of T-SOD, CAT, and GSH levels. The myocardial protection of ginsenoside Rg1 during H/R is partially due to its antioxidative effect and intracellular calcium homeostasis. J. Cell. Biochem. 108: 117-124, 2009. (C) 2009 Wiley-Liss, Inc.

引用

页码：117 / 124

页数：8

共 45 条

[1]

AEBI H, 1984, METHOD ENZYMOL, V105, P121

[2]

Boston DR, 1998, J PHARMACOL EXP THER, V285, P716

[3] Cyclosporine A regulate oxidative stress-induced apoptosis in cardiomyocytes:: mechanisms via ROS generation, iNOS and Hsp70 [J].

Chen, HW ;

Chien, CT ;

Yu, SL ;

Lee, YT ;

Chen, WJ .

BRITISH JOURNAL OF PHARMACOLOGY, 2002, 137 (06) :771-781

[4] Ginsenoside Rg1 attenuates dopamine-induced apoptosis in PC12 cells by suppressing oxidative stress [J].

Chen, XC ;

Zhu, YG ;

Zhu, LA ;

Huang, C ;

Chen, Y ;

Chen, LM ;

Fang, F ;

Zhou, YC ;

Zhao, CH .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2003, 473 (01) :1-7

[5] Ginsenoside Rg1 delays tert-butyl hydroperoxide-induced premature senescence in human WI-38 diploid fibroblast cells [J].

Chen, Xiaochun ;

Zhang, Jing ;

Fang, Yaxiu ;

Zhao, Chaohui ;

Zhu, Yuangui .

JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 2008, 63 (03) :253-264

[6] Anti-amnestic and anti-aging effects of ginsenoside Rg1 and Rb1 and its mechanism of action [J].

Cheng, Y ;

Shen, LH ;

Zhang, JT .

ACTA PHARMACOLOGICA SINICA, 2005, 26 (02) :143-149

[7]

DAS DK, 1994, METHOD ENZYMOL, V233, P601

[8] Role of oxidative stress in cardiovascular diseases [J].

Dhalla, NS ;

Temsah, RM ;

Netticadan, T .

JOURNAL OF HYPERTENSION, 2000, 18 (06) :655-673

[9] FREE-RADICALS AND THEIR INVOLVEMENT DURING LONG-TERM MYOCARDIAL-ISCHEMIA AND REPERFUSION [J].

DOWNEY, JM .

ANNUAL REVIEW OF PHYSIOLOGY, 1990, 52 :487-504

[10] Intracellular signaling by reactive oxygen species during hypoxia in cardiomyocytes [J].

Duranteau, J ;

Chandel, NS ;

Kulisz, A ;

Shao, ZH ;

Schumacker, PT .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (19) :11619-11624

← 1 2 3 4 5 →