Chemically sensitive 3D imaging at sub 100 nm spatial resolution using tomography in a scanning transmission x-ray microscope

被引：0
作者：
Johansson, Goran A. [1 ]
Dynes, James J. [1 ]
Hitchcock, Adam P. [1 ]
Tyliszczak, Tolek [2 ]
Swerhone, George D. W. [3 ]
Lawrence, John R. [3 ]
机构：
[1] McMaster Univ, BIMR, Hamilton, ON L8S 4M1, Canada
[2] LBNL, Berkeley, CA 94720 USA
[3] NWRI, Saskatoon, SK S7N 3H5, Canada
来源：
DEVELOPMENTS IN X-RAY TOMOGRAPHY V | 2006年 / 6318卷
基金：
加拿大自然科学与工程研究理事会;
关键词：
3D chemical imaging; scanning transmission x-ray microscopy; biofilms; spectroscopy; tomography;
D O I：
10.1117/12.681217
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Computed angle scan tomography has been implemented for the first time using sample raster scanning in a scanning transmission X-ray microscope. The experimental apparatus, acquisition and data analysis procedures, and first results from fully hydrated river biofilm samples measured from 528-534 eV are reported. The multiple energy images are processed to 3-dimensional quantitative chemical maps of major biological components, such as proteins, polysaccharides and lipids.
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页数：6
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