Amelioration of myocarditis by HVEM-overexpressing dendritic cells through induction of IL-10-producing cells

被引：17

作者：

Cai, Gang ^{[1
]}

Wang, Huaizhou ^{[1
]}

Qin, Qin ^{[1
]}

Zhang, Jun ^{[1
]}

Zhu, Zhi ^{[2
]}

Liu, Menglei ^{[1
]}

Shen, Qian ^{[1
]}

机构：

[1] Shanghai Changhai Hosp, Dept Expt Diag, Shanghai 200433, Peoples R China

[2] Shanghai Changhai Hosp, Dept Pathol, Shanghai 200433, Peoples R China

来源：

CARDIOVASCULAR RESEARCH | 2009年 / 84卷 / 03期

关键词：

Experimental autoimmune myocarditis; Herpes virus entry mediator; Immune regulation; Dendritic cells; IL-10; HERPESVIRUS ENTRY MEDIATOR; EXPERIMENTAL AUTOIMMUNE MYOCARDITIS; T-LYMPHOCYTE ATTENUATOR; B-LYMPHOCYTE; ACTIVATION; BTLA; INHIBITION; RECEPTOR;

D O I：

10.1093/cvr/cvp219

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Herpes virus entry mediator (HVEM) is considered to be a molecular 'switch' for immune responses, and a role in immune modification has been reported. The aim of this study was to assess whether HVEM-mediated immune suppression could protect against experimental autoimmune myocarditis (EAM) induced by myosin. We constructed HVEM-expressing adenovirus (AdHVEM) and fusion protein HVEM-Ig and evaluated their roles in immunoregulation in vitro and in vivo. Immunoregulation of dendritic cells (DCs) infected with recombinant virus or treated with HVEM-Ig was then studied. DCs transfected with AdHVEM (DC-AdHVEM) were protected against EAM, whereas HVEM-Ig had no protective effect. Further study showed that DC-AdHVEMs produced a regulatory cytokine, IL-10, which had further effects on induction of IL-10 producing CD4(+) T cells. This subset of T cells was then responsible for the protection against EAM. Myosin-DC-AdHVEM cell gene therapy appears to be a safe and effective way of inhibiting the development of EAM. The signal induced by HVEM seems to play different roles in different cells.

引用

页码：425 / 433

页数：9

共 29 条

[1]

Afanasyeva M., AUTOIMMUN REV, V3, P476

[2]

CAI G, J MOL CELL CARDIOL, V38, P593

[3]

CHEUNG TC, P NATL ACAD SCI US, V102, P13218

[4]

DETREZ C, CYTOKINE GROWTH FACT, V19, P277

[5]

FELDMAN AM, N ENGL J MED, V343, P1388

[6]

GAVRIELI M, ADV IMMUNOL, V92, P157

[7]

GONZALEZ LC, P NATL ACAD SCI US, V102, P1116

[8]

GREENWALD RJ, ANN REV IMMUNOL, V23, P151

[9]

HERSKOWITZ A, J AM COLL CARDIOL, V9, P1311

[10]

HODGE JW, FRONT BIOSCI, V11, P788

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