Histone Variant H2AL2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells

被引：110

作者：

Barral, Sophie ^{[1
]}

Morozumi, Yuichi ^{[1
,2
]}

Tanaka, Hiroki ^{[2
]}

Montellier, Emilie ^{[1
]}

Govin, Jerome ^{[3
]}

de Dieuleveult, Maud ^{[4
]}

Charbonnier, Guillaume ^{[5
]}

Coute, Yohann ^{[3
]}

Puthier, Denis ^{[5
]}

Buchou, Thierry ^{[1
]}

Boussouar, Faycal ^{[1
]}

Urahama, Takashi ^{[2
]}

Fenaille, Francois ^{[6
]}

Curtet, Sandrine ^{[1
]}

Hery, Patrick ^{[4
]}

Fernandez-Nunez, Nicolas ^{[5
]}

Shiota, Hitoshi ^{[1
]}

Gerard, Matthieu ^{[4
]}

Rousseaux, Sophie ^{[1
]}

Kurumizaka, Hitoshi ^{[2
]}

Khochbin, Saadi ^{[1
]}

机构：

[1] Univ Grenoble Alpes, Inst Adv Biosci, CNRS,UMR 5309, Inserm,U1209, F-38700 Grenoble, France

[2] Waseda Univ, Grad Sch Adv Sci & Engn, Inst Medoriented Struct Biol, Res Inst Sci & Engn,Lab Struct Biol,Shinjuku Ku, 2-2 Wakamatsu Cho, Tokyo 1628480, Japan

[3] Univ Grenoble Alpes, CEA, Inserm,U1038, BIG BGE, F-38000 Grenoble, France

[4] Univ Paris Saclay, Univ Paris Sud, CNRS, CEA,Inst Integrat Biol Cell I2BC, F-91198 Gif Sur Yvette, France

[5] Aix Marseille Univ, Inserm,U1090, TAGC, TGML,Platform IbiSA, F-13288 Marseille, France

[6] CEA Saclay, DSV IBiTec S SPI, Lab Etud Metabol Med, F-91191 Gif Sur Yvette, France

来源：

MOLECULAR CELL | 2017年 / 66卷 / 01期

关键词：

FUNCTIONAL-PROPERTIES; NUCLEAR PROTEINS; STRUCTURAL BASIS; NUCLEOSOME; PURIFICATION; SPERM; CHAPERONE; H2A.Z;

D O I：

10.1016/j.molcel.2017.02.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Histone replacement by transition proteins (TPs) and protamines (Prms) constitutes an essential step for the successful production of functional male gametes, yet nothing is known on the underlying functional interplay between histones, TPs, and Prms. Here, by studying spermatogenesis in the absence of a spermatid-specific histone variant, H2A.L.2, we discover a fundamental mechanism involved in the transformation of nucleosomes into nucleoprotamines. H2A.L.2 is synthesized at the same time as TPs and enables their loading onto the nucleosomes. TPs do not displace histones but rather drive the recruitment and processing of Prms, which are themselves responsible for histone eviction. Altogether, the incorporation of H2A.L.2 initiates and orchestrates a series of successive transitional states that ultimately shift to the fully compacted genome of the mature spermatozoa. Hence, the current view of histone-to-nucleoprotamine transition should be revisited and include an additional step with H2A.L.2 assembly prior to the action of TPs and Prms.

引用

页码：89 / +

页数：21

共 35 条

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