Quantitative imaging of cellular adhesion by total internal reflection holographic microscopy

被引：36
|
作者：
Ash, William M., III [1 ]
Krzewina, Leo [1 ]
Kim, Myung K. [1 ]
机构：
[1] Univ S Florida, Dept Phys, Digital Holog & Microscopy Lab, Tampa, FL 33620 USA
来源：
APPLIED OPTICS | 2009年 / 48卷 / 34期
基金：
美国国家科学基金会;
关键词：
ANGULAR SPECTRUM METHOD; DIGITAL INTERFERENCE HOLOGRAPHY; TILTED PLANES; PHASE-CONTRAST; RECONSTRUCTION; CELLS;
D O I：
10.1364/AO.48.00H144
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Total internal reflection (TIR) holographic microscopy uses a prism in TIR as a near-field imager to perform quantitative phase microscopy of cell-substrate interfaces. The presence of microscopic organisms, cell-substrate interfaces, adhesions, and tissue structures on the prism's TIR face causes relative index of refraction and frustrated TIR to modulate the object beam's evanescent wave phase front. We present quantitative phase images of test specimens such as Amoeba proteus and cells such as SKOV-3 and 3T3 fibroblasts. (C) 2009 Optical Society of America
引用
收藏
页码：H144 / H152
页数：9
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