Increased apoptotic cells in bone marrow biopsies from patients with aplastic anaemia

被引：31

作者：

Callera, F ^{[1
]}

Falcao, RP ^{[1
]}

机构：

[1] DEPT CLIN MED,SCH MED,BR-14049900 RIBEIRAO PRET,SP,BRAZIL

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1997年 / 98卷 / 01期

关键词：

aplastic anaemia; apoptosis; bone marrow biopsy; stem cells; TUNEL method;

D O I：

10.1046/j.1365-2141.1997.1532971.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In order to investigate the involvement of apoptosis in the pathogenesis of aplastic anaemia (AA) we determined the proportion of apoptotic cells in paraffin-embedded bone marrow biopsies from patients with aplastic anaemia using an in situ TdT-catalysed DNA nick end labelling (TUNEL) staining method. A significant increase in the proportion of mononuclear apoptotic cells was demonstrated in biopsies from patients with aplastic anaemia (8.19 +/- 1.45%) when compared with controls (2.07 +/- 0 86%). These data support the view that apoptosis may play a role in the pathophysiology of bone marrow failure.

引用

页码：18 / 20

页数：3

共 10 条

[1]

Callera F., 1995, Blood, V86, p478A

[2]

CAMITTA BM, 1976, BLOOD, V48, P63

[3]

GAVRIELI Y, 1992, J CELL BIOL, V119, P93

[4] THE POLYPEPTIDE ENCODED BY THE CDNA FOR HUMAN CELL-SURFACE ANTIGEN FAS CAN MEDIATE APOPTOSIS [J].

ITOH, N ;

YONEHARA, S ;

ISHII, A ;

YONEHARA, M ;

MIZUSHIMA, S ;

SAMESHIMA, M ;

HASE, A ;

SETO, Y ;

NAGATA, S .

CELL, 1991, 66 (02) :233-243

[5] APOPTOSIS - BASIC BIOLOGICAL PHENOMENON WITH WIDE-RANGING IMPLICATIONS IN TISSUE KINETICS [J].

KERR, JFR ;

WYLLIE, AH ;

CURRIE, AR .

BRITISH JOURNAL OF CANCER, 1972, 26 (04) :239-+

[6] INCREASED EXPRESSION OF FAS ANTIGEN ON BONE-MARROW CD34(+) CELLS OF PATIENTS WITH APLASTIC-ANEMIA [J].

MACIEJEWSKI, JP ;

SELLERI, C ;

SATO, T ;

ANDERSON, S ;

YOUNG, NS .

BRITISH JOURNAL OF HAEMATOLOGY, 1995, 91 (01) :245-252

[7]

Philpott NJ, 1995, EXP HEMATOL, V23, P1642

[8] A RAPID METHOD FOR MEASURING APOPTOSIS AND DUAL-COLOR IMMUNOFLUORESCENCE BY SINGLE LASER FLOW-CYTOMETRY [J].

SCHMID, I ;

UITTENBOGAART, CH ;

KELD, B ;

GIORGI, JV .

JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 170 (02) :145-157

[9] APOPTOSIS IN THE PATHOGENESIS AND TREATMENT OF DISEASE [J].

THOMPSON, CB .

SCIENCE, 1995, 267 (5203) :1456-1462

[10] HEMATOPOIETIC COLONY STIMULATING FACTORS PROMOTE CELL-SURVIVAL BY SUPPRESSING APOPTOSIS [J].

WILLIAMS, GT ;

SMITH, CA ;

SPOONCER, E ;

DEXTER, TM ;

TAYLOR, DR .

NATURE, 1990, 343 (6253) :76-79

← 1 →