Boronic Acid Functionalized Core-Satellite Composite Nanoparticles for Advanced Enrichment of Glycopeptides and Glycoproteins

被引:137
作者
Zhang, Lijuan [1 ,2 ]
Xu, Yawei [1 ,2 ]
Yao, Hailiang [1 ,2 ]
Xie, Liqi [1 ,2 ]
Yao, Jun [1 ,2 ]
Lu, Haojie [1 ,2 ]
Yang, Pengyuan [1 ,2 ]
机构
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
[2] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China
基金
中国国家自然科学基金;
关键词
enrichment; glycopeptides; glycoproteins; mass spectrometry; nanoparticles; TANDEM MASS-SPECTROMETRY; MAGNETIC NANOPARTICLES; PROTEIN GLYCOSYLATION; RESONANCE; SHELL; DRUG; MOLECULES; CAPTURE; PROBES;
D O I
10.1002/chem.200901347
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A core-satellite-structured composite material has been successfully synthesized for capturing glycosylated peptides or proteins. This novel hybrid material is composed of a silica-coated ferrite "core" and numerous "satellites" of gold nanoparticles with lots of "anchors". The anchor 3-amino-phenylboronic acid. designed for cap luring target molecules, is highly specific toward glycosylated specie. The long organic chains bridging the gold surface and the anchors could reduce the steric hindrance among the bound molecules and suppress nonspecific bindings. Due to the excellent structure of the current material, the trap-and-release enrichment of glycosylated samples is quite simple, specific. and effective Indeed, the composite nanoparticles could be used for enriching glycosylated peptides and proteins with very low concentrations. and the enriched samples can be easily separated from bulk solution by it magnet. By using this strategy. the recovery of glycopeptides and glycoproteins after enrichment were found to be 55.9 and 71.6% separately, whereas the adsorption capacity of the composite nanoparticles was proven to be more than 79 mg, of glycoproteins per gram of the material. Moreover, the new composite nanoparticles were applied to enrich glycosylated proteins from human colorectal cancer tissues for identification of N-glycosylation sites. In all. 194 unique glycosylation sites mapped to 155 different glycoproteins have been identified. of which 165 sites (85.1%) were newly identified.
引用
收藏
页码:10158 / 10166
页数:9
相关论文
共 53 条
[1]  
[Anonymous], 2006, ANGEW CHEM, DOI DOI 10.1002/ange.200602471
[2]  
[Anonymous], 2008, ANGEW CHEM INT EDIT, DOI DOI 10.1002/ANGE.200802469
[3]   Biocompatible heterostructured nanoparticles for multimodal biological detection [J].
Choi, Jin-sil ;
Jun, Young-wook ;
Yeon, Soo-In ;
Kim, Hyoung Chan ;
Shin, Jeon-Soo ;
Cheon, Jinwoo .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (50) :15982-15983
[4]  
CUMMINGS RD, 1982, J BIOL CHEM, V257, P1235
[5]   Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology [J].
Daniel, MC ;
Astruc, D .
CHEMICAL REVIEWS, 2004, 104 (01) :293-346
[6]   Monodisperse magnetic single-crystal ferrite microspheres [J].
Deng, H ;
Li, XL ;
Peng, Q ;
Wang, X ;
Chen, JP ;
Li, YD .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (18) :2782-2785
[7]  
*EBI, INT PROT IND IPI HUM
[8]   Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].
Elghanian, R ;
Storhoff, JJ ;
Mucic, RC ;
Letsinger, RL ;
Mirkin, CA .
SCIENCE, 1997, 277 (5329) :1078-1081
[9]  
*GE HEALTHC, IMAGEMASTER 2D PLAT
[10]   PREPARATION AND CHARACTERIZATION OF AU COLLOID MONOLAYERS [J].
GRABAR, KC ;
FREEMAN, RG ;
HOMMER, MB ;
NATAN, MJ .
ANALYTICAL CHEMISTRY, 1995, 67 (04) :735-743