Glu-Phe from onion (Allium Cepa L.) attenuates lipogenesis in hepatocytes

被引：10

作者：

Lee, Yu Geon ^{[1
,2
]}

Cho, Jeong-Yong ^{[3
]}

Hwang, Eom Ji ^{[3
]}

Jeon, Tae-Il ^{[4
]}

Moon, Jae-Hak ^{[1
,2
]}

机构：

[1] Chonnam Natl Univ, Dept Food Sci & Technol, Plus Program BK21, Gwangju, South Korea

[2] Chonnam Natl Univ, Funct Food Res Ctr, Plus Program BK21, Gwangju, South Korea

[3] Rural Dev Adm, Natl Inst Crop Sci, Bioenergy Crop Res Ctr, Muan, South Korea

[4] Chonnam Natl Univ, Dept Anim Sci, Gwangju, South Korea

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2017年 / 81卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

onion; dipeptide; lipogenesis; SREBP-1c; hepatocytes; FATTY LIVER-DISEASE; ACTIVATED PROTEIN-KINASE; LIPID-ACCUMULATION; INSULIN-RESISTANCE; METABOLIC SYNDROME; CELLS; AMPK; STEATOHEPATITIS; INHIBITION; STEATOSIS;

D O I：

10.1080/09168451.2017.1303358

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A Glu-Phe (EF) was isolated from onion (Allium cepa L. cv. Sunpower). The chemical structure of EF was determined by nuclear magnetic resonance and electrospray ionization-mass (ESI-MS) spectroscopy. We showed that EF reduced lipid accumulation in mouse hepatocytes by inhibiting the expression of sterol regulatory element-binding protein-1c (SREBP-1c) and its lipogenic target genes. We also found that AMP-activated protein kinase (AMPK) was required for the inhibitory effect of EF on lipid accumulation in mouse hepatocytes. Furthermore, EF was qualified in nine onion cultivars by selective multiple reaction-monitoring detection of liquid chromatography-ESI-MS. These results suggest that EF could contribute to the beneficial effect of onion supplement in maintaining hepatic lipid homeostasis.

引用

页码：1409 / 1416

页数：8

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