Rotation of single live mammalian cells using dynamic holographic optical tweezers

被引：33

作者：

Cao, Bin ^{[1
]}

Kelbauskas, Laimonas ^{[1
]}

Chan, Samantha ^{[1
]}

Shetty, Rishabh M. ^{[1
]}

Smith, Dean ^{[1
]}

Meldrum, Deirdre R. ^{[1
]}

机构：

[1] Arizona State Univ, Biodesign Inst, Ctr Biosignatures Discovery Automat, 1001 S McAllister Ave, Tempe, AZ 85287 USA

来源：

OPTICS AND LASERS IN ENGINEERING | 2017年 / 92卷

关键词：

Laser trapping; Optical tweezers; Optical manipulation; Single cells rotation; Spatial light modulation; LIVING CELLS; PARTICLES; MICROMANIPULATION; MANIPULATION; TRACKING; BACTERIA;

D O I：

10.1016/j.optlaseng.2016.12.019

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report on a method for rotating single mammalian cells about an axis perpendicular to the optical system axis through the imaging plane using dynamic holographic optical tweezers (HOTs). Two optical traps are created on the opposite edges of a mammalian cell and are continuously transitioned through the imaging plane along the circumference of the cell in opposite directions, thus providing the torque to rotate the cell in a controlled fashion. The method enables a complete 360 rotation of live single mammalian cells with spherical or near-to spherical shape in 3D space, and represents a useful tool suitable for the single cell analysis field, including tomographic imaging.

引用

页码：70 / 75

页数：6

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