Rotation of single live mammalian cells using dynamic holographic optical tweezers

被引：33

作者：

Cao, Bin ^{[1
]}

Kelbauskas, Laimonas ^{[1
]}

Chan, Samantha ^{[1
]}

Shetty, Rishabh M. ^{[1
]}

Smith, Dean ^{[1
]}

Meldrum, Deirdre R. ^{[1
]}

机构：

[1] Arizona State Univ, Biodesign Inst, Ctr Biosignatures Discovery Automat, 1001 S McAllister Ave, Tempe, AZ 85287 USA

来源：

OPTICS AND LASERS IN ENGINEERING | 2017年 / 92卷

关键词：

Laser trapping; Optical tweezers; Optical manipulation; Single cells rotation; Spatial light modulation; LIVING CELLS; PARTICLES; MICROMANIPULATION; MANIPULATION; TRACKING; BACTERIA;

D O I：

10.1016/j.optlaseng.2016.12.019

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report on a method for rotating single mammalian cells about an axis perpendicular to the optical system axis through the imaging plane using dynamic holographic optical tweezers (HOTs). Two optical traps are created on the opposite edges of a mammalian cell and are continuously transitioned through the imaging plane along the circumference of the cell in opposite directions, thus providing the torque to rotate the cell in a controlled fashion. The method enables a complete 360 rotation of live single mammalian cells with spherical or near-to spherical shape in 3D space, and represents a useful tool suitable for the single cell analysis field, including tomographic imaging.

引用

页码：70 / 75

页数：6

共 34 条

[1] OPTICAL TRAPPING AND MANIPULATION OF VIRUSES AND BACTERIA
ASHKIN, A
DZIEDZIC, JM
[J]. SCIENCE, 1987, 235 (4795) : 1517 - 1520
[2] OPTICAL TRAPPING AND MANIPULATION OF SINGLE CELLS USING INFRARED-LASER BEAMS
ASHKIN, A
DZIEDZIC, JM
YAMANE, T
[J]. NATURE, 1987, 330 (6150) : 769 - 771
[3] Barroso Pella A., 2012, P SOC PHOTO-OPT INS, V8427
[4] Absence of a barrier to backwards rotation of the bacterial flagellar motor demonstrated with optical tweezers
Berry, RM
Berg, HC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (26) : 14433 - 14437
[5] Fiber-optic spanner
Black, Bryan J.
Mohanty, Samarendra K.
[J]. OPTICS LETTERS, 2012, 37 (24) : 5030 - 5032
[6] Rotation of single bacterial cells relative to the optical axis using optical tweezers
Carmon, G.
Feingold, M.
[J]. OPTICS LETTERS, 2011, 36 (01) : 40 - 42
[7] Cellular manipulations in microvortices
Chiu, Daniel T.
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 387 (01) : 17 - 20
[8] Mechanics of the human red blood cell deformed by optical tweezers
Dao, M
Lim, CT
Suresh, S
[J]. JOURNAL OF THE MECHANICS AND PHYSICS OF SOLIDS, 2003, 51 (11-12) : 2259 - 2280
[9] Fully dynamic multiple-beam optical tweezers
Eriksen, RL
Daria, VR
Glückstad, J
[J]. OPTICS EXPRESS, 2002, 10 (14): : 597 - 602
[10] Three-dimensional imaging of single isolated cell nuclei using optical projection tomography
Fauver, M
Seibel, EJ
Rahn, JR
Meyer, MG
Patten, FW
Neumann, T
Nelson, AC
[J]. OPTICS EXPRESS, 2005, 13 (11): : 4210 - 4223

← 1 2 3 4 →