Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements

被引：12

作者：

Gaetani, Chiara ^{[1
]}

Ambrosi, Emmanuele ^{[1
]}

Ugo, Paolo ^{[1
]}

Moretto, Ligia M. ^{[1
]}

机构：

[1] Univ Ca Foscari Venice, Dept Mol Sci & Nanosyst, I-30174 Venice, Italy

来源：

CHEMOSENSORS | 2017年 / 5卷 / 01期

关键词：

immunoglobulin Y; egg yolk; nanoelectrodes ensembles; electrochemical immunosensor; food supplements; SDS-PAGE; HENS EGG-YOLK; IMMUNOGLOBULIN DETECTION; NANOELECTRODE ENSEMBLES; ANTIBODIES; PROTEINS; PURIFICATION;

D O I：

10.3390/chemosensors5010010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Immunoglobulin Y is a water-soluble protein present in high concentration in hen serum and egg yolk. IgY has applications in many fields, e.g., from food stuff to the mass production of antibodies. In this work, we have implemented an electrochemical immunosensor for IgY based on templated nanoelectrodes ensembles. IgY is captured by the templating polycarbonate and reacted with anti-IgY labeled with horseradish peroxidase. In the presence of H2O2 and methylene blue as the redox mediator, an electrocatalytic current is generated which scales with IgY concentration in the sample. After optimizing the extracting procedure, the immunosensor was applied for analysis of fresh eggs and food integrators. The data obtained with the biosensor were validated by SDS-PAGE and Western blot measurements.

引用

页数：11

共 24 条

[1] Production of specific IgY Helicobacter pylori recombinant OipA protein and assessment of its inhibitory effects towards attachment of H. pylori to AGS cell line [J].