Structural determination of the O-antigenic polysaccharide from Escherichia coli O166

被引：10

作者：

Ali, Tara

Weintraub, Andrej

Widmalm, Goeran ^{[1
]}

机构：

[1] Univ Stockholm, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden

[2] Karolinska Univ Hosp, Karolinska Inst, Dept Lab Med, Div Clin Bacteriol, S-14186 Huddinge, Sweden

来源：

CARBOHYDRATE RESEARCH | 2007年 / 342卷 / 02期

关键词：

Escherichia coli; lipopolysaccharide; NMR; biological repeating unit;

D O I：

10.1016/j.carres.2006.11.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The O-antigen of the lipopolysaccharide from Escherichia coli O166 has been determined by component analysis together with ID and 2D NMR spectroscopy techniques. The polysaccharide has pentasaccharide repeating units consisting Of D-glucose (1), D-galactose (2) and N-acetyl-D-galactosamine (2) with the following structure: [GRAPHICS] In the H-1 NMR, spectrum resonances of low intensity were observed. Further analysis of these showed that they originate from the terminal part of the polysaccharide, thereby revealing that the repeating unit has a 3-substituted N-acetyl-D-galactosamine residue at its reducing end. (c) 2006 Elsevier Ltd. All rights reserved.

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收藏

页码：274 / 278

页数：5

相关论文

共 27 条

[1] Serotype-converting bacteriophages and O-antigen modification in Shigella flexneri [J].

Allison, GE ;

Verma, NK .

TRENDS IN MICROBIOLOGY, 2000, 8 (01) :17-23

[2] H-1 AND C-13 ASSIGNMENTS FROM SENSITIVITY-ENHANCED DETECTION OF HETERONUCLEAR MULTIPLE-BOND CONNECTIVITY BY 2D MULTIPLE QUANTUM NMR [J].

BAX, A ;

SUMMERS, MF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (08) :2093-2094

[3] MOLECULAR CHARACTERIZATION OF ENTEROTOXIGENIC ESCHERICHIA-COLI (ETEC) ISOLATED IN NEW-CALEDONIA (VALUE OF POTENTIAL PROTECTIVE ANTIGENS IN ORAL VACCINE CANDIDATES) [J].

BEGAUD, E ;

MONDET, D ;

GERMANI, Y .

RESEARCH IN MICROBIOLOGY, 1993, 144 (09) :721-728

[4] Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolates from healthy sheep in Spain [J].

Blanco, M ;

Blanco, JE ;

Mora, A ;

Rey, J ;

Alonso, JM ;

Hermoso, M ;

Hermoso, J ;

Alonso, MP ;

Dahbi, G ;

González, EA ;

Bernárdez, MI ;

Blanco, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (04) :1351-1356

[5] COHERENCE TRANSFER BY ISOTROPIC MIXING - APPLICATION TO PROTON CORRELATION SPECTROSCOPY [J].

BRAUNSCHWEILER, L ;

ERNST, RR .

JOURNAL OF MAGNETIC RESONANCE, 1983, 53 (03) :521-528

[6] RAPID AND SIMPLE APPROACH FOR THE NMR RESONANCE ASSIGNMENT OF THE CARBOHYDRATE CHAINS OF AN INTACT GLYCOPROTEIN - APPLICATION OF GRADIENT-ENHANCED NATURAL-ABUNDANCE H-1-C-13 HSQC AND HSQC-TOCSY TO THE ALPHA-SUBUNIT OF HUMAN CHORIONIC-GONADOTROPIN [J].

DEBEER, T ;

VANZUYLEN, CEM ;

HARD, K ;

BOELENS, R ;

KAPTEIN, R ;

KAMERLING, JP ;

VLIEGENTHART, JFG .

FEBS LETTERS, 1994, 348 (01) :1-6

[7] Characterization of Shiga toxin-producing Escherichia coli isolated from aquatic environments [J].

García-Aljaro, C ;

Muniesa, M ;

Blanco, JE ;

Blanco, M ;

Blanco, J ;

Jofre, J ;

Blanch, AR .

FEMS MICROBIOLOGY LETTERS, 2005, 246 (01) :55-65

[8] Invited review:: Prevalence of Shiga toxin-producing Escherichia coli in dairy cattle and their products [J].

Hussein, HS ;

Sakuma, T .

JOURNAL OF DAIRY SCIENCE, 2005, 88 (02) :450-465

[9] COMPUTER-ASSISTED STRUCTURAL-ANALYSIS OF POLYSACCHARIDES WITH AN EXTENDED VERSION OF CASPER USING H-1-NMR AND C-13-NMR DATA [J].

JANSSON, PE ;

KENNE, L ;

WIDMALM, G .

CARBOHYDRATE RESEARCH, 1989, 188 :169-191

[10] A TWO-DIMENSIONAL NUCLEAR OVERHAUSER ENHANCEMENT (2D NOE) EXPERIMENT FOR THE ELUCIDATION OF COMPLETE PROTON-PROTON CROSS-RELAXATION NETWORKS IN BIOLOGICAL MACROMOLECULES [J].

KUMAR, A ;

ERNST, RR ;

WUTHRICH, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1980, 95 (01) :1-6