Phenolic contents and antioxidant activity of ethanolic extract of Capparis spinosa

被引：47

作者：

Ben Mansour, Riadh ^{[1
]}

Jilani, Imtinen Ben Haj ^{[2
]}

Bouaziz, Mohammed ^{[3
]}

Gargouri, Bochra ^{[1
]}

Elloumi, Nesrine ^{[1
]}

Attia, Hamadi ^{[1
]}

Ghrabi-Gammar, Zeineb ^{[2
]}

Lassoued, Saloua ^{[1
]}

机构：

[1] Univ Sfax, Inst Super Biotechnol Sfax, Grp Rech Biotechnol & Pathol, Lab Analyse Valorisat & Secur Aliments, Sfax 3038, Tunisia

[2] Inst Natl Agron Tunisie, Fac Lettres, Unite Rech Biogeog Climatol Appl & Dynam Eros, Sfax, Tunisia

[3] Univ Sfax, Ctr Biotechnol Sfax, Lab Bioproc Environm, Sfax 3038, Tunisia

来源：

CYTOTECHNOLOGY | 2016年 / 68卷 / 01期

关键词：

Caper (Capparis spinosa); Phenolic compounds; Ethanol extract; Antioxidant activity; Lipid peroxidation (LPO); L; ANTHOCYANIN; DEGRADATION; PLANTS;

D O I：

10.1007/s10616-014-9764-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Caper plant (Capparis spinosa) extracts have been associated with diverse biological activities including anti-oxidant properties. In this work, we characterized the hydro-ethanolic extract obtained from C. spinosa leaves [hydroethanolic extract of C. spinosa (HECS)] by analyzing the content in anti-oxidant compounds such as polyphenols, flavonoids and anthocyanins. Further, we evaluated HECS antioxidant activities in vitro using bleaching of 1,1-diphenyl-2-picrylhydrazyl radical and ABTS test as well as by pretreatment of HeLa cells exposed to Fe2+ or H2O2. Our findings indicate that HECS contains high amount of total phenolic compounds and high levels of flavonoids and anthocyanins. Furthermore, HECS exhibited antioxidant activity in both chemical and biological tests. Specially, pretreatment of HeLa cells with different concentrations of the extract conferred protection against lipid peroxidation and modulated activities of two antioxidant enzymes, SOD and catalase. These results revealed HECS antioxidant effects and suggest that C. spinosa leaves are a potential source of natural antioxidant molecules with possible applications in industry and medicine.

引用

页码：135 / 142

页数：8

共 24 条

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