A resorbable calcium-deficient hydroxyapatite hydrogel composite for osseous regeneration

被引:31
作者
Hutchens, Stacy A. [2 ,4 ]
Benson, Roberto S. [3 ]
Evans, Barbara R. [4 ]
Rawn, Claudia J. [3 ,6 ]
O'Neill, Hugh [1 ,5 ]
机构
[1] Oak Ridge Natl Lab, Div Chem Sci, Ctr Struct Mol Biol, Oak Ridge, TN 37831 USA
[2] Univ Tennessee, Dept Mech Aerosp & Biomed Engn, Knoxville, TN 37996 USA
[3] Univ Tennessee, Dept Mat Sci & Engn, Knoxville, TN 37996 USA
[4] Oak Ridge Natl Lab, Mol Biosci & Biotechnol Grp, Div Chem Sci, Oak Ridge, TN 37831 USA
[5] Univ Tennessee, Dept Biochem Cellular & Mol Biol, Knoxville, TN 37996 USA
[6] Oak Ridge Natl Lab, Div Met & Ceram, Oak Ridge, TN 37831 USA
基金
美国国家科学基金会;
关键词
Bacterial cellulose; Hydrogel; Hydroxyapatite composite; Oxidized; Resorbable; DIALDEHYDE CELLULOSE; BACTERIAL CELLULOSE; OXIDIZED CELLULOSE; IN-VIVO; BIOCOMPATIBILITY; COTTON; GRAFT; CELLS; PULP;
D O I
10.1007/s10570-009-9300-6
中图分类号
TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];
学科分类号
0805 ; 080502 ; 0822 ;
摘要
It was previously discovered that the unique structure and chemistry of bacterial cellulose (BC) permits the formation of calcium-deficient hydroxyapatite (CdHAP) nanocrystallites under aqueous conditions at ambient pH and temperature. In this study, BC was chemically modified via a limited periodate oxidation reaction to render the composite degradable and thus more suitable for bone regeneration. While native BC does not degrade in mammalian systems, periodate oxidation yields dialdehyde cellulose which breaks down at physiological pH. The composite was characterized by tensile testing, X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy. X-ray diffraction showed that oxidized BC retains its structure and could biomimetically form CdHAP. Degradation behavior was analyzed by incubating the samples in simulated physiological fluid (pH 7.4) at 37 A degrees C under static and dynamic conditions. The oxidized BC and oxidized BC-CdHAP composites both lost significant mass after exposure to the simulated physiological environment. Examination of the incubation solutions by UV-Vis spectrophotometric analysis demonstrated that, while native BC released only small amounts of soluble cellulose fragments, oxidized cellulose releases carbonyl containing degradation products as well as soluble cellulose fragments. By entrapping CdHAP in a degradable hydrogel carrier, this composite should elicit bone regeneration then resorb over time to be replaced by new osseous tissue.
引用
收藏
页码:887 / 898
页数:12
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