Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium?

被引:275
作者
Clemente, M. [2 ]
de la Fuente, J. [2 ]
Fair, T. [1 ]
Al Naib, A. [1 ]
Gutierrez-Adan, A. [2 ]
Roche, J. F. [1 ]
Rizos, D. [2 ]
Lonergan, P. [1 ]
机构
[1] Univ Coll Dublin, Sch Agr Food Sci & Vet Med, Dublin 4, Ireland
[2] INIA, Dept Anim Reprod, Madrid 28040, Spain
基金
爱尔兰科学基金会;
关键词
IN-VITRO; BOVINE EMBRYOS; DAIRY-COWS; DEVELOPMENTAL COMPETENCE; BEEF HEIFERS; SURVIVAL; EXPRESSION; RECEPTOR; MATURATION; PREGNANCY;
D O I
10.1530/REP-09-0152
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The steroid hormone progesterone (P-4) plays a key role in the reproductive events associated with pregnancy establishment and maintenance. High concentrations of circulating P-4 in the immediate post-conception period have been associated with an advancement of conceptus elongation, an associated increase in interferon-tau production and higher pregnancy rates in cattle. Using in vitro and in vivo models and similar to 8500 bovine oocytes across six experiments, the aim of this study was to establish the route through which P-4 affects bovine embryo development in vitro and in vivo. mRNA for P-4 receptors was present at all stages of embryo development raising the possibility of a direct effect of P-4 on the embryo. Exposure to P-4 in vitro in the absence or presence of oviduct epithelial cells did not affect the proportion of embryos developing to the blastocyst stage, blastocyst cell number or the relative abundance of selected transcripts in the blastocyst. Furthermore, exposure to P-4 in vitro did not affect post-hatching elongation of the embryo following transfer to synchronized recipients and recovery on Day 14. By contrast, transfer of in vitro derived blastocysts to a uterine environment previously primed by elevated P-4 resulted in a fourfold increase in conceptus length on Day 14. These data provide clear evidence to support the hypothesis that P-4-induced changes in the uterine environment are responsible for the advancement in conceptus elongation reported previously in cattle and that, interestingly, the embryo does not need to be present during the period of high P-4 in order to exhibit advanced elongation. Reproduction (2009) 138 507-517
引用
收藏
页码:507 / 517
页数:11
相关论文
共 42 条
[1]  
ASHWORTH CJ, 1989, J REPROD FERTIL, V87, P23, DOI 10.1530/jrf.0.0870023
[2]   Embryo-induced transcriptome changes in bovine endometrium reveal species-specific and common molecular markers of uterine receptivity [J].
Bauersachs, Stefan ;
Ulbrich, Susanne E. ;
Gross, Karin ;
Schmidt, Susanne E. M. ;
Meyer, Heinrich H. D. ;
Wenigerkind, Hendrik ;
Vermehren, Margarete ;
Sinowatz, Fred ;
Blum, Helmut ;
Wolf, Eckhard .
REPRODUCTION, 2006, 132 (02) :319-331
[3]   Epigenetic differences between male and female bovine blastocysts produced in vitro [J].
Bermejo-Alvarez, P. ;
Rizos, D. ;
Rath, D. ;
Lonergan, P. ;
Gutierrez-Adan, A. .
PHYSIOLOGICAL GENOMICS, 2008, 32 (02) :264-272
[4]   Effect of increasing progesterone concentration from Day 3 of pregnancy on subsequent embryo survival and development in beef heifers [J].
Carter, F. ;
Forde, N. ;
Duffy, P. ;
Wade, M. ;
Fair, T. ;
Crowe, M. A. ;
Evans, A. C. O. ;
Kenny, D. A. ;
Roche, J. F. ;
Lonergan, P. .
REPRODUCTION FERTILITY AND DEVELOPMENT, 2008, 20 (03) :368-375
[5]   Reproductive functions of progesterone receptors [J].
Conneely, OM ;
Mulac-Jericevic, B ;
DeMayo, F ;
Lydon, JP ;
O'Malley, BW .
RECENT PROGRESS IN HORMONE RESEARCH, VOL 57, 2002, 57 :339-355
[6]   Embryonic and early foetal losses in cattle and other ruminants [J].
Diskin, M. G. ;
Morris, D. G. .
REPRODUCTION IN DOMESTIC ANIMALS, 2008, 43 :260-267
[7]   Quantitative expression of candidate genes for developmental competence in bovine two-cell embryos [J].
Dode, MAN ;
Dufort, I ;
Massicotte, L ;
Sirard, MA .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2006, 73 (03) :288-297
[8]   Embryo and foetal loss in beef heifers between day 14 of gestation and full term [J].
Dunne, LD ;
Diskin, MG ;
Sreenan, JM .
ANIMAL REPRODUCTION SCIENCE, 2000, 58 (1-2) :39-44
[9]   Large-scale transcriptional analysis of bovine embryo biopsies in relation to pregnancy success after transfer to recipients [J].
El-Sayed, Ashraf ;
Hoelker, Michael ;
Rings, Franca ;
Salilew, Dessie ;
Jennen, Danyel ;
Tholen, Ernst ;
Sirard, Marc-Andre ;
Schellander, Karl ;
Tesfaye, Dawit .
PHYSIOLOGICAL GENOMICS, 2006, 28 (01) :84-96
[10]   Progesterone regulation of human granulosa/luteal cell viability by an RU486-independent mechanism [J].
Engmann, Lawrence ;
Losel, Ralf ;
Wehling, Martin ;
Peluso, John J. .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2006, 91 (12) :4962-4968