The many faces of radiation-induced changes

被引：46
作者：
Borek, Dominika
Ginell, Stephan L.
Cymborowski, Marcin
Minor, Wladek
Otwinowski, Zbyszek
机构：
[1] Univ Texas, SW Med Ctr, Dept Biochem, Dallas, TX 75390 USA
[2] Argonne Natl Lab, Struct Biol Ctr, Argonne, IL 60439 USA
[3] Univ Virginia, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22908 USA
来源：
JOURNAL OF SYNCHROTRON RADIATION | 2007年 / 14卷
关键词：
radiation damage; radiation decay; radiation-induced changes; phasing; data collection strategy; PROTEIN CRYSTALS; CRYOGENIC TEMPERATURES; SYNCHROTRON-RADIATION; STRUCTURAL-CHANGES; DAMAGE; CRYSTALLOGRAPHY; DIFFRACTION; ATOMS; LIMIT;
D O I：
10.1107/S0909049506046589
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
During diffraction experiments even cryo-cooled protein crystals can be significantly damaged due to chemical and physical changes induced by absorbed X-ray photons. The character and scale of the observed effects depend strongly on the temperature and the composition of crystals. The absorption of radiation energy results in incremental regular changes to the crystal structure, making its impact on the process of solving the structure strongly correlated with other experimental variables. An understanding of all the dependencies is still limited and does not allow for a precise prediction of the outcome of a particular diffraction experiment. Results are presented of diffraction experiments performed under different experimental conditions. The influence of temperature and crystal composition on different characteristics of radiation damage is analyzed. The observed effects are discussed in terms of their impact on data processing and phasing procedures.
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页码：24 / 33
页数：10
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