nirK-Harboring Denitrifiers Are More Responsive to Denitrification-Inducing Conditions in Rice Paddy Soil Than nirS-Harboring Bacteria

被引：84

作者：

Yoshida, Megumi ^{[1
]}

Ishii, Satoshi ^{[1
]}

Otsuka, Shigeto ^{[1
]}

Senoo, Keishi ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan

来源：

MICROBES AND ENVIRONMENTS | 2010年 / 25卷 / 01期

基金：

日本学术振兴会;

关键词：

denitrification; rice paddy soil; nitrite reductase; nirS; nirK; COMMUNITY STRUCTURE; GENE FRAGMENTS; NOSZ GENES; FOREST; FERTILIZER; DIVERSITY; EMISSION; TIME; N2O; PCR;

D O I：

10.1264/jsme2.ME09160

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We analyzed the quantity and diversity of nitrite reductase genes (nirS and nirK) in rice paddy soil before and after inducing denitrification. A quantitative PCR analysis showed that the copy number of nirK, but not nirS, increased significantly in response to the denitrification-inducing conditions. Diverse nirS and nirK clones were identified by clone library analyses. Clones related to the NirS of Burkholderiales and Rhodocyclales, and NirK distantly related to known denitrifiers increased their proportion in response to the denitrification-inducing conditions, and therefore, might be involved in denitrification in rice paddy soil.

引用

页码：45 / 48

页数：4

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