Changes of thioredoxin and nitric oxide synthase mRNA expression of hippocampus by ischemia and reperfusion in stroke-prone rats

To examine the mechanism of apoptosis by ischemia and reperfusion in SHRSP, neuronal and inducible nitric oxide synthase (eNOS and iNOS), Bcl-2, Bcl-xs, cytochrome oxidase (Cyt-O), thioredoxin-l (TRX-1) and thioredoxin-2 (TRX-2) mRNA were assessed in the hippocampus of SHRSP and WKY by the method of quantitative PCR. Neuronal and inducible NOS, Bcl-xs and Cyt-O mRNA expression in SHRSP were significantly increased after cerebral ischemia and reperfusion. These alterations of NOS mRNA expression might be related to the severer apoptosis in hippocampal neurons reported previously in SHRSP.