In Vivo Measurement of Neurochemical Abnormalities in the Hippocampus in a Rat Model of Cuprizone-Induced Demyelination

被引：6

作者：

Lee, Do-Wan ^{[1
]}

Kwon, Jae-Im ^{[2
]}

Woo, Chul-Woong ^{[2
]}

Heo, Hwon ^{[3
]}

Kim, Kyung Won ^{[1
]}

Woo, Dong-Cheol ^{[2
,3
]}

Kim, Jeong Kon ^{[1
]}

Lee, Dong-Hoon ^{[4
]}

机构：

[1] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Radiol, Seoul 05505, South Korea

[2] Asan Med Ctr, Asan Inst Life Sci, Convergence Med Res Ctr, Seoul 05505, South Korea

[3] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Convergence Med, Seoul 05505, South Korea

[4] Yonsei Univ, Dept Radiat Convergence Engn, Wonju 26493, South Korea

来源：

DIAGNOSTICS | 2021年 / 11卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

rat brain; demyelination; hippocampus; metabolites; multiple sclerosis;

D O I：

10.3390/diagnostics11010045

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This study quantitatively measured the changes in metabolites in the hippocampal lesions of a rat model of cuprizone-induced demyelination as detected using in vivo 7 T proton magnetic resonance spectroscopy. Nineteen Sprague Dawley rats were randomly divided into two groups and fed a normal chow diet or cuprizone (0.2%, w/w) for 7 weeks. Demyelinated hippocampal lesions were quantitatively measured using a 7 T magnetic resonance imaging scanner. All proton spectra were quantified for metabolite concentrations and relative ratios. Compared to those in the controls, the cuprizone-induced rats had significantly higher concentrations of glutamate (p = 0.001), gamma-aminobutyric acid (p = 0.019), and glutamate + glutamine (p = 0.001); however, creatine + phosphocreatine (p = 0.006) and myo-inositol (p = 0.001) concentrations were lower. In addition, we found that the glutamine and glutamate complex/total creatine (p < 0.001), glutamate/total creatine (p < 0.001), and GABA/total creatine (p = 0.002) ratios were significantly higher in cuprizone-treated rats than in control rats. Our results showed that cuprizone-induced neuronal demyelination may influence the severe abnormal metabolism in hippocampal lesions, and these responses could be caused by microglial activation, mitochondrial dysfunction, and astrocytic necrosis.

引用

页数：10

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