Effects of nicotine and vitamin E on glutathione reductase activity in some rat tissues in vivo and in vitro

被引:40
作者
Erat, Mustafa
Ciftci, Mehmet [1 ]
Gumustekin, Kenan
Gul, Mustafa
机构
[1] Ataturk Univ, Arts & Sci Fac, Dept Chem, TR-25240 Erzurum, Turkey
[2] Ataturk Univ, Biotechnol Applicat & Res Ctr, TR-25240 Erzurum, Turkey
[3] Ataturk Univ, Fac Med, Dept Physiol, TR-25240 Erzurum, Turkey
关键词
glutathione reductase; nicotine; rat tissues; vitamin E;
D O I
10.1016/j.ejphar.2006.10.008
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Effects of nicotine, and nicotine + vitamin E on glutathione reductase (Glutathione: NADP(+) oxidoreductase, EC 1.8.1.7) activity in the muscle, heart, lungs, testicles, kidney, stomach, brain and liver tissues were investigated in vivo and also in vitro. The groups were: nicotine [0.5 mg/kg/day, intraperitoneal (i.p.)]; nicotine + vitamin E [75 mg/kg/day, intragastric (i.g.)]; and control group (receiving only vehicles). There were eight rats per group and supplementation period was 3 weeks. The results showed that nicotine (0.5 mg/kg, i.p.) inhibited glutathione reductase activity significantly in the liver, lungs, heart, stomach, kidney, and testicles by similar to 61.5%, similar to 65%, similar to 70.5%, similar to 72.5%, similar to 64% and similar to 71.5%, respectively, while it had activated glutathione reductase activity in the brain by similar to 11.8%, and had no effect on the muscle glutathione reductase activity. Vitamin E supplementation prevented this nicotine-induced decrease in glutathione reductase activity in liver, lungs, heart, stomach, and kidney. However, it did not prevent this nicotine-induced decrease in testicles. In vitro studies were also carried out to elucidate the effects of nicotine and vitamin E on glutathione reductase activity. In vitro results correlated well with in vivo experimental results in liver, lungs, heart, stomach, and testicular tissues. These results show that vitamin E administration generally restores the inactivation of glutathione reductase activity due to nicotine administration in various rat tissues in vivo, and also in vitro. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:92 / 97
页数:6
相关论文
共 67 条
[1]   Impact of adolescent nicotine exposure on adenylyl cyclase-mediated cell signaling:: enzyme induction, neurotransmitter-specific effects, regional selectivities, and the role of withdrawal [J].
Abreu-Villaça, Y ;
Seidler, FJ ;
Slotkin, TA .
BRAIN RESEARCH, 2003, 988 (1-2) :164-172
[2]   In vitro effects of some drugs on catalase purified from human skin [J].
Altikat, Sayit ;
Coban, Abdulkadir ;
Ciftci, Mehmet ;
Ozdemir, Hasan .
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 2006, 21 (02) :231-234
[3]   OXIDANTS, ANTIOXIDANTS, AND THE DEGENERATIVE DISEASES OF AGING [J].
AMES, BN ;
SHIGENAGA, MK ;
HAGEN, TM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :7915-7922
[4]   THE CHRONIC ADMINISTRATION OF NICOTINE INDUCES CYTOCHROME-P450 IN RAT-BRAIN [J].
ANANDATHEERTHAVARADA, HK ;
WILLIAMS, JF ;
WECKER, L .
JOURNAL OF NEUROCHEMISTRY, 1993, 60 (05) :1941-1944
[5]  
[Anonymous], US DEP HHS PUBL
[6]  
ASHAKUMARY L, 1991, J ECOTOXICOL ENV MON, V1, P283
[7]  
Beutler, 1984, RED CELL METABOLISM
[8]   Effects of some medical drugs on enzyme activities of carbonic anhydrase from human erythrocytes in vitro and from rat erythrocytes in vivo [J].
Beydemir, S ;
Çiftçi, M ;
Özmen, I ;
Buyukokuroglu, ME ;
Özdemir, H ;
Küfrevioglu, ÖI .
PHARMACOLOGICAL RESEARCH, 2000, 42 (02) :187-191
[9]   Preferential effects of nicotine and 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone on mitochondrial glutathione S-transferase A4-4 induction and increased oxidative stress in the rat brain [J].
Bhagwat, SV ;
Vijayasarathy, C ;
Raza, H ;
Mullick, J ;
Avadhani, NG .
BIOCHEMICAL PHARMACOLOGY, 1998, 56 (07) :831-839
[10]  
BONDY SC, 1994, ALCOHOL ALCOHOLISM, V29, P375