Simultaneous real-time analysis of Paneth cell and intestinal stem cell response to interferon-γ by a novel stem cell niche tracking method

被引：11

作者：

Yokoi, Yuki ^{[1
]}

Adachi, Takahiro ^{[2
]}

Sugimoto, Rina ^{[3
]}

Kikuchi, Mani ^{[1
]}

Ayabe, Tokiyoshi ^{[1
,3
]}

Nakamura, Kiminori ^{[1
,3
]}

机构：

[1] Hokkaido Univ, Fac Adv Life Sci, Dept Cell Biol Sci, Innate Immun Lab,Kita Ku, Kita 21,Nishi 11, Sapporo, Hokkaido 0010021, Japan

[2] Tokyo Med & Dent Univ, Med Res Inst, Dept Immunol, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138510, Japan

[3] Hokkaido Univ, Grad Sch Life Sci, Innate Immun Lab, Kita Ku, Kita 21,Nishi 11, Sapporo, Hokkaido 0010021, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2021年 / 545卷

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Paneth cell; Intestinal stem cell; Stem cell niche; Interferon-gamma; Live imaging;

D O I：

10.1016/j.bbrc.2021.01.050

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Paneth cells and Lgr5+ intestinal stem cells (Lgr5+ ISCs) constitute the stem cell niche and maintain small intestinal epithelial integrity by recognizing various niche factors derived from subepithelial cells and external antigens. Although it has been known that interferon-gamma (IFN-gamma), a Th1 cytokine, is associated with intestinal epithelial disruption during inflammation as a niche factor, dynamics of Paneth cells and Lgr5+ ISCs in response to IFN-gamma remain to be understood. Here we show that CAG-tdTomato;Lgr5-EGFP (CT-LE) mice generated in this study enable to identify Paneth cells and Lgr5+ ISCs separately by fluorescence signals. Lgr5+ ISCs underwent cell death a little earlier than Paneth cells in response to IFN-gamma by simultaneous tracking using CT-LE mice. In addition, the timing of cell death in most Paneth cells overlapped with Lgr5+ ISCs, suggesting that Paneth cell depletion is induced directly by IFN-gamma. Taken together, we established a novel simultaneous stem cell niche tracking method and clarified the involvement of both Paneth cells and Lgr5+ ISCs in stem cell niche damage induced by IFN-gamma, further contribute to understanding the mechanism for maintaining intestinal homeostasis by stem cell niche. (C) 2021 The Authors. Published by Elsevier Inc.

引用

页码：14 / 19

页数：6

共 28 条

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