Functional optoacoustic neuro-tomography of calcium fluxes in adult zebrafish brain in vivo

被引：16
作者：
Dean-Ben, X. Luis [1 ]
Gottschalk, Sven [1 ]
Sela, Gali [1 ]
Shoham, Shy [2 ]
Razansky, Daniel [1 ,3 ]
机构：
[1] Helmholtz Ctr Munich, IBMI, Neuherberg, Germany
[2] Technion Israel Inst Technol, Dept Biomed Engn, Haifa, Israel
[3] Tech Univ Munich, Sch Med, D-80290 Munich, Germany
来源：
OPTICS LETTERS | 2017年 / 42卷 / 05期
基金：
欧洲研究理事会; 美国国家卫生研究院;
关键词：
ENCODED OPTICAL INDICATORS; FLUORESCENT PROTEINS; MICROSCOPY; NETWORKS; CIRCUITS;
D O I：
10.1364/OL.42.000959
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Genetically-encoded calcium indicators (GECIs) have revolutionized neuroimaging by enabling mapping of the activity of entire neuronal populations in vivo. Visualization of these powerful activity sensors has to date been limited to depth-restricted microscopic studies due to intense light scattering in the brain. We demonstrate, for the first time, in vivo real-time volumetric optoacoustic monitoring of calcium transients in adult transgenic zebrafish expressing the GCaMP5G calcium indicator. Fast changes in optoacoustic traces associated with GCaMP5G activity were detectable in the presence of other strongly absorbing endogenous chromophores, such as hemoglobin. The new functional optoacoustic neuroimaging method can visualize neural activity at penetration depths and spatio-temporal resolution scales not covered with the existing neuroimaging techniques. (C) 2017 Optical Society of America
引用
收藏
页码：959 / 962
页数：4
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