Pleiotropic effects of negative energy balance in the postpartum dairy cow on splenic gene expression: repercussions for innate and adaptive immunity

被引：48

作者：

Morris, D. G. ^{[1
]}

Waters, S. M. ^{[2
]}

McCarthy, S. D. ^{[1
,3
]}

Patton, J.

Earley, B. ^{[2
]}

Fitzpatrick, R. ^{[1
]}

Murphy, J. J. ^{[4
]}

Diskin, M. G. ^{[1
]}

Kenny, D. A. ^{[3
]}

Brass, A. ^{[5
]}

Wathes, D. C. ^{[6
]}

机构：

[1] TEAGASC, Athenry, Co Galway, Ireland

[2] TEAGASC, Grange, Meath, Ireland

[3] Univ Coll Dublin, Sch Agr Food Sci & Vet Med, Dublin, Ireland

[4] TEAGASC, Moorepk, Cork, Ireland

[5] Univ Manchester, Sch Life Sci, Manchester, Lancs, England

[6] Univ London Royal Vet Coll, London, England

来源：

PHYSIOLOGICAL GENOMICS | 2009年 / 39卷 / 01期

基金：

英国惠康基金;

关键词：

nonesterified fatty acids; butyrate; spleen; ENDOPLASMIC-RETICULUM STRESS; PLASMINOGEN-ACTIVATOR RECEPTOR; UNCOUPLING PROTEIN-2; FATTY-ACIDS; LIVER; BIOLOGY; INTERLEUKIN-15; IDENTIFICATION; NETWORKS; CELLS;

D O I：

10.1152/physiolgenomics.90394.2008

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Morris DG, Waters SM, McCarthy SD, Patton J, Earley B, Fitzpatrick R, Murphy JJ, Diskin MG, Kenny DA, Brass A, Wathes DC. Pleiotropic effects of negative energy balance in the postpartum dairy cow on splenic gene expression: repercussions for innate and adaptive immunity. Physiol Genomics 39: 28-37, 2009. First published June 30, 2009; doi: 10.1152/physiolgenomics.90394.2008.-Increased energy demands to support lactation, coupled with lowered feed intake capacity results in negative energy balance (NEB) and is typically characterized by extensive mobilization of body energy reserves in the early postpartum dairy cow. The catabolism of stored lipid leads to an increase in the systemic concentrations of nonesterified fatty acids (NEFA) and beta-hydroxy butyrate (BHB). Oxidation of NEFA in the liver result in the increased production of reactive oxygen species and the onset of oxidative stress and can lead to disruption of normal metabolism and physiology. The immune system is depressed in the peripartum period and early lactation and dairy cows are therefore more vulnerable to bacterial infections causing mastitis and or endometritis at this time. A bovine Affymetrix oligonucleotide array was used to determine global gene expression in the spleen of dairy cows in the early postpartum period. Spleen tissue was removed post mortem from five severe NEB (SNEB) and five medium NEB (MNEB) cows 15 days postpartum. SNEB increased systemic concentrations of NEFA and BHB, and white blood cell and lymphocyte numbers were decreased in SNEB animals. A total of 545 genes were altered by SNEB. Network analysis using Ingenuity Pathway Analysis revealed that SNEB was associated with NRF2-mediated oxidative stress, mitochondrial dysfunction, endoplasmic reticulum stress, natural killer cell signaling, p53 signaling, down-regulation of IL-15, BCL-2, and IFN-gamma; upregulation of BAX and CHOP and increased apoptosis with a potential negative impact on innate and adaptive immunity.

引用

页码：28 / 37

页数：10

共 85 条

[81] Nrf2 and Nrf1 in association with Jun proteins regulate antioxidant response element-mediated expression and coordinated induction of genes encoding detoxifying enzymes [J].