A competitive ELISA for detection of group specific antibody to bluetongue virus using anti-core antibody

被引：7
作者：
Chand, Karam [1 ]
Biswas, Sanchay K. [1 ]
Pandey, Awadh B. [1 ]
Saxena, Arpit [1 ]
Tewari, Neha [1 ]
Mondal, Bimalendu [2 ]
机构：
[1] ICAR Indian Vet Res Inst, Div Virol, Mukteswar Campus, Mukteshwar 263138, Uttarakhand, India
[2] ICAR Indian Vet Res Inst, Eastern Reg Stn, 37 Belgachia Rd, Kolkata 700037, W Bengal, India
来源：
BIOLOGICALS | 2017年 / 46卷
关键词：
C-ELISA; Bluetongue virus; Serogroup; VP7; protein; VP7;
D O I：
10.1016/j.biologicals.2017.01.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Bluetongue virus (BTV) is transmitted by biting midges, which infects domestic and wild ruminants. In present study, a competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of serogroup-specific antibodies against VP7 protein of BTV has been developed. The assay measures the competition between a group specific antibody against core protein of BTV and a test serum to an optimized concentration of BTV recombinant-VP7 (r-VP7) antigen. Serum samples (n = 895) collected from small and large ruminants were used to optimize the C-ELISA. Percent inhibition (PI) values were used for estimation of the cut-off value for the C-ELISA. On receiver operator characteristic (ROC) analysis, different cut-off values along with their diagnostic sensitivity (DSn) and diagnostic specificity (DSp) were obtained. Among these, >50% PI value was accepted as cut-off at which DSn and Dsp was achieved as 97.6% and 98.0% respectively, at >95% confidence interval. Results show the present C-ELISA assay described to be sensitive, specific and reliable and could be adopted for serological investigation of small and large ruminants. (C) 2017 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：168 / 171
页数：4
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