Radiation-Induced Dual Oxidase Upregulation in Rat Heart Tissues: Protective Effect of Melatonin

被引：39

作者：

Farhood, Bagher ^{[1
,2
]}

Aliasgharzadeh, Akbar ^{[1
,2
]}

Amini, Peyman ^{[3
]}

Saffar, Hana ^{[4
]}

Motevaseli, Elahe ^{[5
]}

Rezapoor, Saeed ^{[3
]}

Nouruzi, Farzad ^{[6
]}

Shabeeb, Dheyauldeen ^{[7
]}

Musa, Ahmed Eleojo ^{[8
]}

Ashabi, Ghorbangol ^{[9
]}

Mohseni, Mehran ^{[1
,2
]}

Moradi, Habiballah ^{[1
,2
]}

Najafi, Masoud ^{[10
]}

机构：

[1] Kashan Univ Med Sci, Fac Paramed Sci, Dept Med Phys, Kashan 8715988141, Iran

[2] Kashan Univ Med Sci, Fac Paramed Sci, Dept Radiol, Kashan 8715988141, Iran

[3] Univ Tehran Med Sci, Fac Paramed, Dept Radiol, Tehran 1416753955, Iran

[4] Univ Tehran Med Sci, Imam Khomeini Hosp Complex, Tehran 1419733141, Iran

[5] Univ Tehran Med Sci, Sch Adv Technol Med, Dept Mol Med, Tehran 1416753955, Iran

[6] Islamic Azad Univ, Sci & Res Branch, Dept Med Radiat Engn, Tehran 1477893855, Iran

[7] Univ Misan, Dept Physiol, Coll Med, Misan 62010, Iraq

[8] Tehran Univ Med Sci Int Campus, Dept Med Phys, Tehran 1416753955, Iran

[9] Univ Tehran Med Sci, Fac Med, Dept Physiol, Tehran 1416753955, Iran

[10] Kermanshah Univ Med Sci, Sch Paramed Sci, Radiol & Nucl Med Dept, Kermanshah 6715847141, Iran

来源：

MEDICINA-LITHUANIA | 2019年 / 55卷 / 07期

关键词：

radiation; melatonin; IL-4; Duox1; Duox2; heart; OXIDATIVE STRESS; IRRADIATION; AMIFOSTINE; DAMAGE; CELLS; LUNG;

D O I：

10.3390/medicina55070317

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Radiation-induced heart injury can lead to increased risk of heart failure, attack, and ischemia. Some studies proposed IL-4 and IL-13 as two important cytokines that are involved in late e ff ects of ionizing radiation. On the other hand, these cytokines may, through upregulation of Duox1 and Duox2, induce chronic oxidative stress, inflammation, and fibrosis. In this study, we evaluated the upregulation of Duox1 and Duox2 pathways in hearts following chest irradiation in rats and then detected possible attenuation of them by melatonin. Materials and Methods: Twenty male Wistar rats were divided into four groups: (1) control; (2) melatonin treated (100 mg / kg); (3) radiation (15 Gy gamma rays); (4) melatonin treated before irradiation. All rats were sacrificed after 10 weeks and their heart tissues collected for real-time PCR (RT-PCR), ELISA detection of IL-4 and IL-13, as well as histopathological evaluation of macrophages and lymphocytes infiltration. Results: Results showed an upregulation of IL-4, IL4ra1, Duox1, and Duox2. The biggest changes were for IL4ra1 and Duox1. Treatment with melatonin before irradiation could attenuate the upregulation of all genes. Melatonin also caused a reduction in IL-4 as well as reverse infiltration of inflammatory cells. Conclusion: Duox1 and Duox2 may be involved in the late e ff ects of radiation-induced heart injury. Also, via attenuation of these genes, melatonin can o ff er protection against the toxic e ff ects of radiation on the heart.

引用

页数：10

共 35 条

[1] NADPH oxidase DUOX1 promotes long-term persistence of oxidative stress after an exposure to irradiation [J].