MicroRNA-510 Plays Oncogenic Roles in Non-Small Cell Lung Cancer by Directly Targeting SRC Kinase Signaling Inhibitor 1

被引:15
作者
Wu, Wei [1 ]
He, Linyan [2 ,3 ]
Huang, Yan [1 ]
Hou, Likun [1 ]
Zhang, Wei [1 ]
Zhang, Liping [1 ]
Wu, Chunyan [1 ]
机构
[1] Tongji Univ, Shanghai Pulm Hosp, Dept Pathol, Sch Med, 507 Zhengmin Rd, Shanghai 200433, Peoples R China
[2] Soochow Univ, Affiliated Hosp 1, Jiangsu Inst Hematol, Key Lab Thrombosis & Hemostasis,Minist Hlth, Suzhou, Peoples R China
[3] Soochow Univ, Collaborat Innovat Ctr Hematol, Suzhou, Peoples R China
关键词
Non-small cell lung cancer (NSCLC); MicroRNA-510; Proliferation; Invasion; SRC kinase signaling inhibitor 1 (SRCIN1); TUMOR-SUPPRESSOR; PROLIFERATION; EXPRESSION; PROMOTES; P140CAP; INVOLVEMENT; CARCINOMA; MIGRATION; INVASION; MIRNAS;
D O I
10.3727/096504018X15451308507747
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
An increasing number of studies have demonstrated that microRNAs (miRNAs) may play key roles in various cancer carcinogenesis and progression, including non-small cell lung cancer (NSCLC). However, the expressions, roles, and mechanisms of miR-510 in NSCLC have, up to now, been largely undefined. In vivo assay showed that miR-510 was upregulated in NSCLC tissues compared with that in adjacent nontumor lung tissues. miR-510 expression was significantly correlated with TNM stage and lymph node metastasis. In vitro assay indicated that expressions of miR-510 were also increased in NSCLC cell lines. Downregulation of miR-510 suppressed NSCLC cell proliferation and invasion in vitro. We identified SRC kinase signaling inhibitor 1 (SRCIN1) as a direct target gene of miR-510 in NSCLC. Expression of SRCIN1 was downregulated in lung cancer cells and negatively correlated with miR-510 expression in tumor tissues. Downregulation of SRCIN1, leading to inhibition of miR-510 expression, reversed cell proliferation and invasion in NSCLC cells. These results showed that miR-510 acted as an oncogenic miRNA in NSCLC, partly by targeting SRCIN1, suggesting that miR-510 can be a potential approach for the treatment of patients with malignant lung cancer.
引用
收藏
页码:879 / 887
页数:9
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