Disposition of gamma-hydroxybutyric acid in conventional and nonconventional biologic fluids after single drug administration:: Issues in methodology and drug monitoring

被引:58
作者
Abanades, Sergio
Farre, Magi
Segura, Mireia
Pichini, Simona
Pastor, Antoni
Pacifici, Roberta
Pellegrini, Manuela
de la Torre, Rafael
机构
[1] IMIM, Pharmacol Res Unit, Human Pharmacol & Clin Neurosci Res Grp, Barcelona, Spain
[2] Ist Super Sanita, Drug Res & Evaluat Dept, I-00161 Rome, Italy
[3] Univ Autonoma Barcelona, E-08193 Barcelona, Spain
[4] Univ Pompeu Fabra, Barcelona, Spain
关键词
GHB; plasma; oral fluid; subjective effects; sweat;
D O I
10.1097/FTD.0b013e3180307e5e
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Little controlled drug administration data are available to aid in the interpretation of gamma-hydroxybutyric acid (GHB) distribution in conventional and nonconventional fluids and the potential correlation between the pharmacokinetics of GHB and drug effects. Single oral sodium GHB doses of 50 mg/kg were administered to five volunteers. Plasma, oral fluid, urine, and sweat were analyzed for GHB by gas chromatography mass spectrometry. GHB stability in plasma was studied at different storage temperatures. Subjective effects were measured using a set of 13 different visual analog scales. Mean peak GHB plasma concentrations at 30 minutes were 83.1 mu g/mL. After the absorption phase, concentrations declined to mean values of 0.9 mu g/mL at 6 hours. GHB was found in oral fluid at peak value concentrations equivalent to one third to one fourth of those found in plasma. The oral fluid-to-plasma ratio varied two fold in the 1- to 6-hour time range but always was lower than unit. The mean half-life (t(1/2)) of GHB was approximately 0.7 hour in plasma and approximately 1.2 hours in oral fluid. GHB urinary excretion is less than 2% of the dose administered. GHB was also detected in sweat at low concentrations. GHB showed a mixed sedative-stimulant pattern with subjective effects peaking between 1 and 1.5 hours after drug administration and lasting for 2 hours. Oral fluid and sweat appeared not to be suitable biologic matrices for monitoring GHB consumption. GHB -mediated subjective effects are related to GHB plasma concentrations.
引用
收藏
页码:64 / 70
页数:7
相关论文
共 37 条
  • [21] In vitro production of gamma-hydroxybutyrate in antemortem urine samples
    Kerrigan, S
    [J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 2002, 26 (08) : 571 - 574
  • [22] Kintz P, 2003, J FORENSIC SCI, V48, P195
  • [23] Kintz P, 2001, CLIN CHEM, V47, P2033
  • [24] KROUWER JS, 1984, CLIN CHEM, V30, P290
  • [25] A comprehensive study on the variations in urinary concentrations of endogenous gamma-hydroxybutyrate (GHB)
    LeBeau, MA
    Montgomery, MA
    Morris-Kukoski, C
    Schaff, JE
    Deakin, A
    Levine, B
    [J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 2006, 30 (02) : 98 - 105
  • [26] Analysis of biofluids for gamma-hydroxybutyrate (GHB) and gamma-butyrolactone (GBL) by headspace GC-FID and GC-MS
    LeBeau, MA
    Montgomery, MA
    Miller, ML
    Burmeister, SG
    [J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 2000, 24 (06) : 421 - 428
  • [27] Analysis of gamma-hydroxybutyrate (GHB) in urine by gas chromatography-mass spectrometry
    McCusker, RR
    Paget-Wilkes, H
    Chronister, CW
    Goldberger, BA
    ElSohly, MA
    [J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 1999, 23 (05) : 301 - 305
  • [28] Navarro M, 2001, CLIN CHEM, V47, P1788
  • [29] GHB: a new and novel drug of abuse
    Nicholson, KL
    Balster, RL
    [J]. DRUG AND ALCOHOL DEPENDENCE, 2001, 63 (01) : 1 - 22
  • [30] PALATINI P, 1993, EUR J CLIN PHARMACOL, V45, P353