An isocratic concurrent assay of free metabolites, 4-hydroxy-3-methoxy mandelic acid, 3-methoxy-4-hydroxy-phenylglycol, normetanephrine, metanephrine, and 5-hydroxy-indoleacetic acid in same sample of urine extract using HPLC-ECD

被引:7
作者
Kumar, AM
Fernandez, JB
Goodkin, K
Schneiderman, N
Eisdorfer, C
机构
[1] UNIV MIAMI,SCH MED,DEPT BEHAV SCI,MIAMI,FL 33101
[2] UNIV MIAMI,SCH MED,BEHAV MED RES CTR,MIAMI,FL 33101
关键词
D O I
10.1080/10826079708005553
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper describes a method for simultaneous extraction and concurrent quantification of metabolites of norepinepohrine (NE) and 5-hydroxytryptamine (5-HT) in human urine using an isocratic HPLC-ECD technique. These metabolites include 4-hydroxy-3-methoxy mandelic acid (VMA), 3-methoxy-4-hydroxyphenylglycol (MHPG), normetanephrine (NMTN), metanephrine (MTN), and 5-hydroxyindoleacetic acid (5-HIAA). The samples of urine were passed through preconditioned alumina-B column and washed with diethylether to remove interfering substances. Metabolites were extracted in ethylacetate.
引用
收藏
页码:1931 / 1943
页数:13
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