SPIRE: The SPIDER Reconstruction Engine

被引:17
作者
Baxter, William T.
Leith, ArDean
Frank, Joachim
机构
[1] Wadsworth Ctr, Albany, NY 12201 USA
[2] Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA
关键词
cryo-electron microscopy; single-particle reconstruction; graphical user interface;
D O I
10.1016/j.jsb.2006.07.019
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
SPIRE is a Python program written to modernize the user interaction with SPIDER, the image processing system for electron microscopical reconstruction projects. SPIRE provides a graphical user interface (GUI) to SPIDER for executing batch files of SPIDER commands. It also lets users quickly view the status of a project by showing the last batch files that were run, as well as the data files that were generated. SPIRE handles the flexibility of the SPIDER programming environment through configuration files: XML-tagged documents that describe the batch files, directory trees, and presentation of the GUI for a given type of reconstruction project. It also provides the capability to connect to a laboratory database, for downloading parameters required by batch files at the start of a project, and uploading reconstruction results at the end of a project. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:56 / 63
页数:8
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