Immobilized anthraquinone for redox mediation of horseradish peroxidase for hydrogen peroxide sensing

被引:20
作者
Dou, Yuehua [1 ,2 ]
Haswell, Stephen [1 ]
Greenman, John [2 ]
Wadhawan, Jay [1 ]
机构
[1] Univ Hull, Dept Phys Sci Chem, Kingston Upon Hull HU6 7RX, N Humberside, England
[2] Univ Hull, Postgrad Med Inst, Biomed Res Ctr, Kingston Upon Hull HU6 7RX, N Humberside, England
关键词
Hydrogen peroxide; Biosensor; Anthraquinone; Horseradish peroxidase; MODIFIED ELECTRODES; CARBON ELECTRODES; REDUCTION; CATALYSIS; SURFACE; HEART; GOLD;
D O I
10.1016/j.elecom.2009.08.032
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
The detection of hydrogen peroxide is detailed using horseradish peroxidase and anthraquinone. Both species are immobilized on a glassy carbon electrode substrate. This dual immobilization gives rise to lower detection limits compared with the situation when either of the species is immobilized. Detection limits of 40 nM are reported within physiologically-relevant media. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:1976 / 1981
页数:6
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