A Raf-like protein kinase BHP mediates blue light-dependent stomatal opening

被引:64
作者
Hayashi, Maki [1 ]
Inoue, Shin-ichiro [1 ]
Ueno, Yoshihisa [1 ,3 ]
Kinoshita, Toshinori [1 ,2 ]
机构
[1] Nagoya Univ, Grad Sch Sci, Div Biol Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan
[2] Nagoya Univ, Inst Transformat Biomol WPI ITbM, Chikusa Ku, Nagoya, Aichi 4648602, Japan
[3] Ryukoku Univ, Dept Agr, Seta Oe Cho, Otsu, Shiga 5202194, Japan
关键词
MEMBRANE H+-ATPASE; PLANT PLASMA-MEMBRANE; GUARD-CELL PROTOPLASTS; VICIA-FABA; BIOCHEMICAL-CHARACTERIZATION; C-TERMINUS; IN-VITRO; PHOSPHORYLATION; ACTIVATION; INTERACTS;
D O I
10.1038/srep45586
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Stomata in the plant epidermis open in response to blue light and affect photosynthesis and plant growth by regulating CO2 uptake and transpiration. In stomatal guard cells under blue light, plasma membrane H+-ATPase is phosphorylated and activated via blue light-receptor phototropins and a signaling mediator BLUS1, and H+-ATPase activation drives stomatal opening. However, details of the signaling between phototropins and H+-ATPase remain largely unknown. In this study, through a screening of specific inhibitors for the blue light-dependent H+-ATPase phosphorylation in guard cells, we identified a Raf-like protein kinase, BLUE LIGHT-DEPENDENT H+-ATPASE PHOSPHORYLATION (BHP). Guard cells in the bhp mutant showed impairments of stomatal opening and H+-ATPase phosphorylation in response to blue light. BHP is abundantly expressed in the cytosol of guard cells and interacts with BLUS1 both in vitro and in vivo. Based on these results, BHP is a novel signaling mediator in blue light-dependent stomatal opening, likely downstream of BLUS1.
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页数:12
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