Determination of Bovine Serum Albumin by a Resonance Light-Scattering Technique with a New Mixed-Complex La(C7H5O5S)(phen)3+

被引：0

作者：

Li, Ling ^{[1
]}

Song, Gongwu ^{[1
]}

Fang, Guangrong ^{[1
]}

机构：

[1] Hubei Univ, Coll Chem & Chem Engn, Wuhan 430062, Peoples R China

来源：

INSTRUMENTATION SCIENCE & TECHNOLOGY | 2009年 / 37卷 / 06期

关键词：

Bovine serum albumin; Mixed complex; Resonance light scattering; PROTEIN ASSAY; ACID; MICRODETERMINATION;

D O I：

10.1080/10739140903252006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new mixed-complex of La(C7H5O5S)(phen)(3+) was synthesized. Resonance light-scattering characteristics of the interaction between La(C7H5O5S)(phen)(3+) with bovine serum albumin (BSA) were studied. After BSA was added, aggregation of La(C7H5O5S)(phen)(3+) on the molecular surface of the bovine serum albumin occurred at the pH 4.5 similar to 6.2, which resulted in an enhanced resonance light-scattering (RLS) peak at 400 nm. The intensity of resonance light-scattering was found to be proportional to the concentration of BSA.

引用

页码：631 / 638

页数：8

共 17 条

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