Changes of sphingolipids profiles after ischemia-reperfusion injury in the rat liver

被引：9

作者：

Zhai Shu-ting ^{[1
]}

Liu Guang-yi ^{[1
]}

Xue Fei ^{[1
]}

Sun Gong-ping ^{[1
]}

Liang Liang ^{[1
]}

Chen Wei ^{[1
]}

Xu Guo-dong ^{[1
]}

Li Jun-jian ^{[1
]}

Yang Jun ^{[1
]}

Liang Ting-bo ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Hepatobiliary & Pancreat Surg, Affiliated Hosp 1,Minist Publ Hlth, Key Lab Combined Multiorgan Transplantat,Sch Med, Hangzhou 310003, Zhejiang, Peoples R China

来源：

CHINESE MEDICAL JOURNAL | 2009年 / 122卷 / 24期

基金：

中国国家自然科学基金;

关键词：

lipidomic; sphingolipids; liver; ischemia-reperfusion; matrix-assisted laser desorption ionization time-of-flight mass spectrometry; NECROSIS-FACTOR-ALPHA; ASSISTED LASER-DESORPTION; MASS-SPECTROMETRY; CELL-DEATH; INDUCED APOPTOSIS; MECHANISMS; CERAMIDE; SPHINGOMYELINASE; ACTIVATION; ISCHEMIA/REPERFUSION;

D O I：

10.3760/cma.j.issn.0366-6999.2009.24.022

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background Hepatic ischemia-reperfusion (I/R) injury occurs in many clinical procedures. The molecular mechanisms responsible for hepatic I/R injury however remain unknown. Sphingolipids, in particular ceramide, play a role in stress and death receptor-induced hepatocellular death, contributing to the progression of several liver diseases including liver I/R injury. In order to further define the role of sphingolipids in hepatic I/R, systemic analysis of sphingolipids after reperfusion is necessary. Methods We investigated the lipidomic changes of sphingolipids in a rat model of warm hepatic I/R injury, by delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry (DE MALDI-TOF-MS). Results The total amounts of ceramide and sphingomyelin and the intensity of most kinds of sphingolipids, mainly sphingomyelin, significantly increased at 1 hour after reperfusion (P<0.05) and reached peaks at 6 hours after reperfusion (P<0.01) compared to controls. Six new forms of ceramide and sphingomyelins appeared 6 hours after reperfusion, they were (m/z) 537.8, 555.7, 567.7, 583.8, 683.5 and 731.4 respectively. A ceramide-monohexoside (m/z) 804.4 (CMH(d18:1C22:1+Na)(+)) also increased after reperfusion and correlated with extent of liver injury after reperfursion. Conclusions Three main forms of sphingolipids, ceramide, sphingomyelin and ceramide-monohexoside, are related to hepatic I/R injury and provide a new perspective in understanding the mechanisms responsible for hepatic I/R injury. Chin Med J 2009;122(24):3025-3031

引用

页码：3025 / 3031

页数：7

共 29 条

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