Albumin modulates the production of matrix metalloproteinases-2 and-9 in podocytes

被引：8
作者：
Fang, Zhan [1 ]
He, Fangfang [1 ]
Chen, Shan [1 ]
Sun, Xifeng [1 ]
Zhu, Zhonghua [1 ]
Zhang, Chun [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Dept Nephrol, Union Hosp, Tongji Med Coll, Wuhan 430022, Peoples R China
来源：
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES | 2009年 / 29卷 / 06期
关键词：
podocytes; albumin; matrix metalloproteinases; glomerular basement membrane; GLOMERULAR-BASEMENT-MEMBRANE; TGF-BETA; MMP-9; COLLAGEN; INJURY; PLAY;
D O I：
10.1007/s11596-009-0607-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To investigate the effects of albumin on the production of matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinases-9 (MMP-9) in podocytes. Podocytes were treated with bovine serum albumin (BSA) at the concentration of 0.1, 0.5, 1, 2 g/L, respectively. Conditioned media were harvested 12, 24, 48 and 72 h after the treatment. The expression of MMP-2 and MMP-9 was assayed by gelatin zymography, RT-PCR and Western blotting analysis. Our results showed that in comparison with the control group, BSA increased the expression of MMP-2 and MMP-9 mRNA and protein in a dose- and time-dependent manner (P < 0.05). Meanwhile, the enzymatic activities of MMP-2 and MMP-9 in the culture supernatants of podocytes were also increased (P < 0.05). It is concluded that albumin up-regulated the expression of MMP-2 and MMP-9 at gene and protein levels in a time- and dose-dependent manner.
引用
收藏
页码：710 / 714
页数：5
相关论文
共 28 条
[11]   Statin-sensitive endocytosis of albumin by glomerular podocytes [J].
Eyre, Jeanette ;
Ioannou, Kyriakos ;
Grubb, Blair D. ;
Saleem, Moin A. ;
Mathieson, Peter W. ;
Brunskill, Nigel J. ;
Christensen, Erik I. ;
Topham, Peter S. .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2007, 292 (02) :F674-F681
[12]  
Fowlkes John L., 1995, Progress in Growth Factor Research, V6, P255, DOI 10.1016/0955-2235(95)00017-8
[13]   Podocyte flattening and disorder of glomerular basement membrane are associated with splitting of dystroglycan-matrix interaction [J].
Kojima, K ;
Davidovits, A ;
Poczewski, H ;
Langer, B ;
Uchida, S ;
Nagy-Bojarski, K ;
Hovorka, A ;
Sedivy, R ;
Kerjaschki, D .
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2004, 15 (08) :2079-2089
[14]   Regulation of adhesive interaction between podocytes and glomerular basement membrane [J].
Kretzler, M .
MICROSCOPY RESEARCH AND TECHNIQUE, 2002, 57 (04) :247-253
[15]  
Kuroda Tadahide, 2004, Clin Exp Nephrol, V8, P206
[16]   Do matrix metalloproteinases MMP-2 and MMP-9 (gelatinases) play a role in renal development, physiology and glomerular diseases? [J].
Lelongt, B ;
Legallicier, B ;
Piedagnel, R ;
Ronco, PN .
CURRENT OPINION IN NEPHROLOGY AND HYPERTENSION, 2001, 10 (01) :7-12
[17]   Podocyte biology in diabetic nephropathy [J].
Li, J. J. ;
Kwak, S. J. ;
Jung, D. S. ;
Kim, J-J ;
Yoo, T-H ;
Ryu, D-R ;
Han, S. H. ;
Choi, H. Y. ;
Lee, J. E. ;
Moon, S. J. ;
Kim, D. K. ;
Han, D. S. ;
Kang, S-W .
KIDNEY INTERNATIONAL, 2007, 72 :S36-S42
[18]   Hypercholesterolaemia induces early renal lesions characterized by upregulation of MMP-9 and iNOS and ETAR: alleviated by a dual endothelin receptor antagonist CPU0213 and simvastatin [J].
Luo, Lu ;
Dai, De-Zai ;
Zheng, Yu-Fen ;
Dai, Yin .
JOURNAL OF PHARMACY AND PHARMACOLOGY, 2009, 61 (06) :775-780
[19]   In response to protein load podocytes reorganize cytoskeleton and modulate endothelin-1 gene - Implication for permselective dysfunction of chronic nephropathies [J].
Morigi, M ;
Buelli, S ;
Angioletti, S ;
Zanchi, C ;
Longaretti, L ;
Zoja, C ;
Galbusera, M ;
Gastoldi, S ;
Mundel, P ;
Remuzzi, G ;
Benigni, A .
AMERICAN JOURNAL OF PATHOLOGY, 2005, 166 (05) :1309-1320
[20]   Urinary excretion of podocytes in patients with diabetic nephropathy [J].
Nakamura, T ;
Ushiyama, C ;
Suzuki, S ;
Hara, M ;
Shimada, N ;
Ebihara, I ;
Koide, H .
NEPHROLOGY DIALYSIS TRANSPLANTATION, 2000, 15 (09) :1379-1383
123