Albumin modulates the production of matrix metalloproteinases-2 and-9 in podocytes

被引：8

作者：

Fang, Zhan ^{[1
]}

He, Fangfang ^{[1
]}

Chen, Shan ^{[1
]}

Sun, Xifeng ^{[1
]}

Zhu, Zhonghua ^{[1
]}

Zhang, Chun ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Dept Nephrol, Union Hosp, Tongji Med Coll, Wuhan 430022, Peoples R China

来源：

JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES | 2009年 / 29卷 / 06期

关键词：

podocytes; albumin; matrix metalloproteinases; glomerular basement membrane; GLOMERULAR-BASEMENT-MEMBRANE; TGF-BETA; MMP-9; COLLAGEN; INJURY; PLAY;

D O I：

10.1007/s11596-009-0607-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the effects of albumin on the production of matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinases-9 (MMP-9) in podocytes. Podocytes were treated with bovine serum albumin (BSA) at the concentration of 0.1, 0.5, 1, 2 g/L, respectively. Conditioned media were harvested 12, 24, 48 and 72 h after the treatment. The expression of MMP-2 and MMP-9 was assayed by gelatin zymography, RT-PCR and Western blotting analysis. Our results showed that in comparison with the control group, BSA increased the expression of MMP-2 and MMP-9 mRNA and protein in a dose- and time-dependent manner (P < 0.05). Meanwhile, the enzymatic activities of MMP-2 and MMP-9 in the culture supernatants of podocytes were also increased (P < 0.05). It is concluded that albumin up-regulated the expression of MMP-2 and MMP-9 at gene and protein levels in a time- and dose-dependent manner.

引用

收藏

页码：710 / 714

页数：5

相关论文

共 28 条

[1] Transforming growth factor-β1 is up-regulated by podocytes in response to excess intraglomerular passage of proteins -: A central pathway in progressive glomerulosclerosis [J].

Abbate, M ;

Zoja, C ;

Morigi, M ;

Rottoli, D ;

Angioletti, S ;

Tomasoni, S ;

Zanchi, C ;

Longaretti, L ;

Donadelli, R ;

Remuzzi, G .

AMERICAN JOURNAL OF PATHOLOGY, 2002, 161 (06) :2179-2193

[2] High ambient glucose levels modulates the production of MMP-9 and α5(IV) collagen by cultured podocytes [J].

Bai, YL ;

Wang, LZ ;

Li, YQ ;

Liu, SY ;

Li, JZ ;

Wang, HY ;

Huang, HC .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2006, 17 (1-2) :57-68

[3] DEGRADATION OF GLOMERULAR BASEMENT-MEMBRANE BY PURIFIED MAMMALIAN METALLOPROTEINASES [J].

BARICOS, WH ;

MURPHY, G ;

ZHOU, YW ;

NGUYEN, HH ;

SHAH, SV .

BIOCHEMICAL JOURNAL, 1988, 254 (02) :609-612

[4] Antioxidant treatment reduces matrix metalloproteinase-2-induced vascular changes in renovascular hypertension [J].

Castro, Michele M. ;

Rizzi, Elen ;

Rodrigues, Gerson J. ;

Ceron, Carla S. ;

Bendhack, Lusiane M. ;

Gerlach, Raquel F. ;

Tanus-Santos, Jose E. .

FREE RADICAL BIOLOGY AND MEDICINE, 2009, 46 (09) :1298-1307

[5] Role of matrix metalloproteinases in renal pathophysiologies [J].

Catania, J. M. ;

Chen, G. ;

Parrish, A. R. .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2007, 292 (03) :F905-F911

[6] Angiotensin II stimulates α3(IV) collagen production in mouse podocytes via TGF-β and VEGF signalling:: implications for diabetic glomerulopathy [J].

Chen, SD ;

Lee, JS ;

Iglesias de la Cruz, MC ;

Wang, A ;

Izquierdo-Lahuerta, A ;

Gandhi, NK ;

Danesh, FR ;

Wolf, G ;

Ziyadeh, FN .

NEPHROLOGY DIALYSIS TRANSPLANTATION, 2005, 20 (07) :1320-1328

[7] Integrin α1β1 regulates matrix metalloproteinases via p38 mitogen-activated protein kinase in mesangial cells -: Implications for Alport syndrome [J].

Cosgrove, Dominic ;

Meehan, Daniel T. ;

Delimont, Duane ;

Pozzi, Ambra ;

Chen, Xiwu ;

Rodgers, Kathyrn D. ;

Tempero, Richard M. ;

Zallocchi, Marisa ;

Rao, Velidi H. .

AMERICAN JOURNAL OF PATHOLOGY, 2008, 172 (03) :761-773

[8] Podocyte injury in focal segmental glomerulosclerosis: Lessons from animal models (a play in five acts) [J].

D'Agati, V. D. .

KIDNEY INTERNATIONAL, 2008, 73 (04) :399-406

[9] Increased plasma metalloproteinase-9 concentrations precede development of microalbuminuria in non-insulin-dependent diabetes mellitus [J].

Ebihara, I ;

Nakamura, T ;

Shimada, N ;

Koide, H .

AMERICAN JOURNAL OF KIDNEY DISEASES, 1998, 32 (04) :544-550

[10] Matrix metalloproteinase-2, matrix metalloproteinase-9, and tissue inhibitor of metalloproteinase-1 in the peripheral blood of patients with various glomerular diseases and their implication in pathogenetic lesions: Study based on an enzyme-linked assay and immunohistochemical staining [J].

Endo T. ;

Nakabayashi K. ;

Sekiuchi M. ;

Kuroda T. ;

Soejima A. ;

Yamada A. .

Clinical and Experimental Nephrology, 2006, 10 (4) :253-261